Isolation of Quinolone Alkaloids from Tetradium ruticarpum via Preparative High-Speed Counter-Current Chromatography and Evaluation of Their Binding Affinities for Bovine Serum Albumin
Zou, HJ (Zou, Huijuan)[ 1 ]; Guo, GH (Guo, Guanghao)[ 1 ]; Wang, ML (Wang, Minglong)[ 1 ]; Cao, JG (Cao, Jianguo)[ 1 ]; Huang, GZ (Huang, Guozheng)[ 1,2 ]
2018
发表期刊ACTA CHROMATOGRAPHICA
ISSN1233-2356
卷号30期号:2页码:73-80
摘要

By application of preparative high-speed counter-current chromatography (HSCCC) to the crude quinolone alkaloids (1.1 g) from the fruit of Tetradium ruticarpum, 1-methyl-2((6Z,9Z)-pentadecadienyl)-4(1H)-quinolone (1, 8.4 mg), dihydroevocarpine (2, 27.0 mg), and 1-methyl-2-pentadecyl-4(1H)-quinolone (3, 18.8 mg) were isolated in one step with sufficient purity using the solvent system composed of hexane-ethyl acetate-methanol-water (Hex-EtOAc-MeOH-H2O, 5: 2: 5: 3). Further purification of the subfraction was performed by amending the solvent composition and achieved another three quinolone alkaloids, i.e., 1-methyl-2-undecylquinolin-4(1H)-one (4, 13.7 mg), (Z)-1-methyl-2-(tridec-5-en-1-yl) quinolin-4(1H)-one (5, 14.0 mg) from subfraction FR3-A3-85 using Hex-EtOAc-MeOH-H2O (5:3.5:8.75:8.25), and 1-methyl-2-nonylquinolin-4(1H)-one (6, 15.1 mg) from subfraction FR3-A3-36 using Hex-EtOAc-MeOH-H2O (5:3.8:5:4.8). The relationship between the structure of the six alkaloids and their affinities for bovine serum albumin (BSA) was investigated using fluorescence titration analysis. The length and the presence of double bond of the side chain affected their binding process with BSA. The binding behavior might influence their other biological activities.

关键词Quinolone Alkaloid Hsccc Binding Affinity Fluorescence Titration Analysis
DOI10.1556/1326.2017.00174
收录类别SCI
WOS记录号WOS:000432268400001
引用统计
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/5608
专题省部共建新疆特有药用资源利用重点实验室
作者单位1.Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 201418, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China
推荐引用方式
GB/T 7714
Zou, HJ ,Guo, GH ,Wang, ML ,et al. Isolation of Quinolone Alkaloids from Tetradium ruticarpum via Preparative High-Speed Counter-Current Chromatography and Evaluation of Their Binding Affinities for Bovine Serum Albumin[J]. ACTA CHROMATOGRAPHICA,2018,30(2):73-80.
APA Zou, HJ ,Guo, GH ,Wang, ML ,Cao, JG ,&Huang, GZ .(2018).Isolation of Quinolone Alkaloids from Tetradium ruticarpum via Preparative High-Speed Counter-Current Chromatography and Evaluation of Their Binding Affinities for Bovine Serum Albumin.ACTA CHROMATOGRAPHICA,30(2),73-80.
MLA Zou, HJ ,et al."Isolation of Quinolone Alkaloids from Tetradium ruticarpum via Preparative High-Speed Counter-Current Chromatography and Evaluation of Their Binding Affinities for Bovine Serum Albumin".ACTA CHROMATOGRAPHICA 30.2(2018):73-80.
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