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Isolation of Quinolone Alkaloids from Tetradium ruticarpum via Preparative High-Speed Counter-Current Chromatography and Evaluation of Their Binding Affinities for Bovine Serum Albumin
Zou, HJ (Zou, Huijuan)[ 1 ]; Guo, GH (Guo, Guanghao)[ 1 ]; Wang, ML (Wang, Minglong)[ 1 ]; Cao, JG (Cao, Jianguo)[ 1 ]; Huang, GZ (Huang, Guozheng)[ 1,2 ]
2018
Source PublicationACTA CHROMATOGRAPHICA
ISSN1233-2356
Volume30Issue:2Pages:73-80
Abstract

By application of preparative high-speed counter-current chromatography (HSCCC) to the crude quinolone alkaloids (1.1 g) from the fruit of Tetradium ruticarpum, 1-methyl-2((6Z,9Z)-pentadecadienyl)-4(1H)-quinolone (1, 8.4 mg), dihydroevocarpine (2, 27.0 mg), and 1-methyl-2-pentadecyl-4(1H)-quinolone (3, 18.8 mg) were isolated in one step with sufficient purity using the solvent system composed of hexane-ethyl acetate-methanol-water (Hex-EtOAc-MeOH-H2O, 5: 2: 5: 3). Further purification of the subfraction was performed by amending the solvent composition and achieved another three quinolone alkaloids, i.e., 1-methyl-2-undecylquinolin-4(1H)-one (4, 13.7 mg), (Z)-1-methyl-2-(tridec-5-en-1-yl) quinolin-4(1H)-one (5, 14.0 mg) from subfraction FR3-A3-85 using Hex-EtOAc-MeOH-H2O (5:3.5:8.75:8.25), and 1-methyl-2-nonylquinolin-4(1H)-one (6, 15.1 mg) from subfraction FR3-A3-36 using Hex-EtOAc-MeOH-H2O (5:3.8:5:4.8). The relationship between the structure of the six alkaloids and their affinities for bovine serum albumin (BSA) was investigated using fluorescence titration analysis. The length and the presence of double bond of the side chain affected their binding process with BSA. The binding behavior might influence their other biological activities.

KeywordQuinolone Alkaloid Hsccc Binding Affinity Fluorescence Titration Analysis
DOI10.1556/1326.2017.00174
Indexed BySCI
WOS IDWOS:000432268400001
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Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/5608
Collection省部共建新疆特有药用资源利用重点实验室
Affiliation1.Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 201418, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China
Recommended Citation
GB/T 7714
Zou, HJ ,Guo, GH ,Wang, ML ,et al. Isolation of Quinolone Alkaloids from Tetradium ruticarpum via Preparative High-Speed Counter-Current Chromatography and Evaluation of Their Binding Affinities for Bovine Serum Albumin[J]. ACTA CHROMATOGRAPHICA,2018,30(2):73-80.
APA Zou, HJ ,Guo, GH ,Wang, ML ,Cao, JG ,&Huang, GZ .(2018).Isolation of Quinolone Alkaloids from Tetradium ruticarpum via Preparative High-Speed Counter-Current Chromatography and Evaluation of Their Binding Affinities for Bovine Serum Albumin.ACTA CHROMATOGRAPHICA,30(2),73-80.
MLA Zou, HJ ,et al."Isolation of Quinolone Alkaloids from Tetradium ruticarpum via Preparative High-Speed Counter-Current Chromatography and Evaluation of Their Binding Affinities for Bovine Serum Albumin".ACTA CHROMATOGRAPHICA 30.2(2018):73-80.
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