Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals' Bone Marrow
Rozi, P (Rozi, Parhat)[ 1,2,3 ]; Maimaiti, P (Maimaiti, Palida)[ 4 ]; Abuduwaili, A (Abuduwaili, Aytursun)[ 1,2,3 ]; Wali, A (Wali, Ahmidin)[ 1,2,3 ]; Yili, A (Yili, Abulimiti)[ 1,2 ]; Aisa, HA (Aisa, Haji Akber)[ 1,2 ]
2018
发表期刊MOLECULES
ISSN1420-3049
卷号23期号:7页码:1-14
摘要

In this work, proteins and peptides were isolated from four kinds of animal bone marrow and characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and liquid chromatography-mass spectrometry (LC/MS). The antimicrobial and antioxidant activity of these proteins were investigated in vitro. The nutritional value was evaluated by analyzing their free amino acid composition. The results indicates that all of the extracts appeared two bands at SDS-PAGE, the peptide band at 4.1-10 kDa and protein band at 66 kDa, these data are consistent with LC/MS results. FT-IR analysis showed that the secondary structure of protein mainly consists of alpha-helix. SEM micrographs revealed that the fractions have different morphological characteristics. Horse bone marrow protein (HBMP) showed the highest antioxidant activity to DPPH free radical, IC50 value was 0.573 mg/mL. Most of the obtained fractions showed antimicrobial activities towards Escherichia coli (EC) and Candida albicans (CA). Total free amino acid content ranged between 5.15-49.60 mg/g, and among them, HBMP displayed the highest abundance, 49.7 mg/g, which amino acid composition ratio approached the Food and Agriculture Organization/World Health Organization (FAO/WHO) ideal amino acid pattern recommendation. This study provides fundamental knowledge and a basic study method for the research into and development of animal bone marrow proteins and peptides as functional food and drug resources.

关键词Bone Marrow Protein And Peptide Nutritional Evaluation Sds-page Lc/ms
DOI10.3390/molecules23071673
收录类别SCI
WOS记录号WOS:000445301800176
引用统计
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/5603
专题省部共建新疆特有药用资源利用重点实验室
资源化学研究室
通讯作者Yili, A (Yili, Abulimiti)[ 1,2 ]
作者单位1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Zone, 40-1 Rd, Urumqi 830011, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
4.Xinjiang Med Univ, Dept Nursing, Urumqi 830011, Peoples R China
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GB/T 7714
Rozi, P ,Maimaiti, P ,Abuduwaili, A ,et al. Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals' Bone Marrow[J]. MOLECULES,2018,23(7):1-14.
APA Rozi, P ,Maimaiti, P ,Abuduwaili, A ,Wali, A ,Yili, A ,&Aisa, HA .(2018).Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals' Bone Marrow.MOLECULES,23(7),1-14.
MLA Rozi, P ,et al."Isolation and Evaluation of Bioactive Protein and Peptide from Domestic Animals' Bone Marrow".MOLECULES 23.7(2018):1-14.
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