Isolation and identification of two potential antioxidant peptides from sheep abomasum protein hydrolysates
Liu, B (Liu, Bing)[ 1,4 ]; Aisa, HA (Aisa, Haji Akber)[ 1,2,3 ]; Yili, A (Yili, Abulimiti)[ 1,2,3 ]
2018
发表期刊EUROPEAN FOOD RESEARCH AND TECHNOLOGY
ISSN1438-2377
卷号244期号:9页码:1615-1625
摘要

The objective of this study was to obtain antioxidant peptides from sheep abomasum protein hydrolysates (SAPH). Sheep abomasum proteins were first hydrolyzed using alcalase, neutrase, or papain. Results from three enzyme tests revealed that the hydrolysis process of papain catalyst was most effective as SAPH generated by papain exhibited the highest degree of hydrolysis (DH), yield, and antioxidant activity. The effect of the conditions for hydrolysis with papain was further optimized using the response surface methodology. The highest DH and yield of SAPH (16.86 and 40.65%, respectively) were obtained under the following conditions: hydrolysis temperature, 46 degrees C; hydrolysis time, 3.8 h; and enzyme/substrate ratio, 1.5%. Two novel peptides with high antioxidant activity were purified from SAPH by ultrafiltration, ion exchange chromatography, gel filtration chromatography, and reverse-phase high-performance liquid chromatography. Peptide sequences were determined as Leu-Glu-Asp-Gly-Leu-Lys (LEDGLK, SAPHP-A) and Ile-Asp-Asp-Val-Leu-Lys (IDDVLK, SAPHP-B) with molecular weights of 674.37 and 703.41 Da, respectively. The results of the present study indicated that peptides purified from SAPH were natural antioxidants and could be used as food additives and pharmaceutical products.

关键词Antioxidant Peptide Sheep Abomasum Enzymatic Hydrolysis Optimization Purification
DOI10.1007/s00217-018-3074-8
收录类别SCI
WOS记录号WOS:000440731100010
引用统计
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/5578
专题省部共建新疆特有药用资源利用重点实验室
资源化学研究室
作者单位1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Beijing South Rd 40-1, Urumqi 830011, Peoples R China
2.Key Lab Plant Resources & Chem Arid Zone, Urumqi, Peoples R China
3.State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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Liu, B ,Aisa, HA ,Yili, A . Isolation and identification of two potential antioxidant peptides from sheep abomasum protein hydrolysates[J]. EUROPEAN FOOD RESEARCH AND TECHNOLOGY,2018,244(9):1615-1625.
APA Liu, B ,Aisa, HA ,&Yili, A .(2018).Isolation and identification of two potential antioxidant peptides from sheep abomasum protein hydrolysates.EUROPEAN FOOD RESEARCH AND TECHNOLOGY,244(9),1615-1625.
MLA Liu, B ,et al."Isolation and identification of two potential antioxidant peptides from sheep abomasum protein hydrolysates".EUROPEAN FOOD RESEARCH AND TECHNOLOGY 244.9(2018):1615-1625.
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