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A Label-Free Electrochemical Aptasensor for the Rapid Detection of Tetracycline Based on Ordered Mesoporous Carbon-Fe3O4
Zhan, XJ (Zhan, Xuejia); Hu, GZ (Hu, Guangzhi); Wagberg, T (Wagberg, Thomas); Zhang, DW (Zhang, Dongwei); Zhou, P (Zhou, Pei)
2018
Source PublicationAUSTRALIAN JOURNAL OF CHEMISTRY
Volume71Issue:2-3Pages:170-176
Abstract

A novel aptasensor based on a tetracycline (TET) aptamer immobilized by physical adsorption on an ordered mesoporous carbon-Fe3O4 (OMC-Fe3O4)-modified screen-printed electrode surface was successfully fabricated. OMC-Fe3O4 was characterized by scanning electron microscopy, X-ray diffraction, and X-ray photoelectron spectroscopy. The modification procedure of the aptasensor was characterized by cyclic voltammetry. Interaction between the TET aptamer and target was determined by differential pulse voltammetry. Under optimal conditions, the proposed aptasensor exhibited good electrochemical sensitivity to TET in a concentration range of 5 nM to 10 mu M, with a detection limit of 0.8 nM (S/N = 3). This aptasensor exhibited satisfactory specificity, reproducibility, and stability.

DOI10.1071/CH17503
Indexed BySCI
WOS IDWOS:000426369100012
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Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/5298
Collection环境科学与技术研究室
Affiliation1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China
2.Shanghai Jiao Tong Univ, Bor S Luh Food Safety Res Ctr, Shanghai 200240, Peoples R China
3.Minist Agr, Key Lab Urban Agr South China, Guangzhou 510640, Guangdong, Peoples R China
4.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Lab Environm Sci & Technol, Urumqi 830011, Peoples R China
5.Umea Univ, Dept Phys, S-90187 Umea, Sweden
Recommended Citation
GB/T 7714
Zhan, XJ ,Hu, GZ ,Wagberg, T ,et al. A Label-Free Electrochemical Aptasensor for the Rapid Detection of Tetracycline Based on Ordered Mesoporous Carbon-Fe3O4[J]. AUSTRALIAN JOURNAL OF CHEMISTRY,2018,71(2-3):170-176.
APA Zhan, XJ ,Hu, GZ ,Wagberg, T ,Zhang, DW ,&Zhou, P .(2018).A Label-Free Electrochemical Aptasensor for the Rapid Detection of Tetracycline Based on Ordered Mesoporous Carbon-Fe3O4.AUSTRALIAN JOURNAL OF CHEMISTRY,71(2-3),170-176.
MLA Zhan, XJ ,et al."A Label-Free Electrochemical Aptasensor for the Rapid Detection of Tetracycline Based on Ordered Mesoporous Carbon-Fe3O4".AUSTRALIAN JOURNAL OF CHEMISTRY 71.2-3(2018):170-176.
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