Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS and the Antioxidant Activity In Vitro
Reheman, A (Reheman, Ayinuer); Aisa, HA (Aisa, Haji Akber); Ma, QL (Ma, Qing Ling); Nijat, D (Nijat, Dilaram); Abdulla, R (Abdulla, Rahima)
2018
发表期刊EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
ISSN1741-427X
卷号2018期号:3页码:1-13
摘要

By merging a high-performance liquid chromatography diode array detector (HPLC-DAD) method with high-performance thin-layer chromatography (HPTLC), an assay was developed for chemical fingerprinting and quantitative analysis of traditional medicine Majun Mupakhi ELA (MME), and constituent compounds were identified using HPLC coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS method. In addition, the antioxidant capacity of MME was assessed based on the ability of components to scavenge radicals using in vitro method. Using a HPLC-DAD method with HPTLC easily validated the chemical fingerprinting results and quantified three characteristic components, namely, gallic acid (1), daidzein (2), and icariin (3), in commercial MMEs. The three compounds presented excellent regression values (R-2 = 0.9999) in the ranges of the test and the method recovery was in the range from 100.49% to 100.68%. The fingerprints had 27 common characteristic peaks, of which 13 were verified by rapid UHPLC-DAD-Q-Orbitrap-MS analysis. In vitro antioxidant assays rapidly assessed and contrasted antioxidant activity or the free radical scavenging activity of the main polyphenolic classes in MMEs, and the antioxidant capacity was mostly affected by the presence of gallic acid. Thus, this study establishes a powerful and meaningful approach for MME quality control and for assessing in vitro antioxidant activity.

DOI10.1155/2018/1035809
收录类别SCI
WOS记录号WOS:000427752800001
引用统计
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/5294
专题省部共建新疆特有药用资源利用重点实验室
资源化学研究室
通讯作者Aisa, HA (Aisa, Haji Akber)
作者单位1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Reg, Urumqi 830011, Xinjiang, Peoples R China
3.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Peoples R China
4.Xinjiang Med Univ, Coll Tradit Uyghur Med, Urumqi 830011, Xinjiang, Peoples R China
推荐引用方式
GB/T 7714
Reheman, A ,Aisa, HA ,Ma, QL ,et al. Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS and the Antioxidant Activity In Vitro[J]. EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2018,2018(3):1-13.
APA Reheman, A ,Aisa, HA ,Ma, QL ,Nijat, D ,&Abdulla, R .(2018).Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS and the Antioxidant Activity In Vitro.EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE,2018(3),1-13.
MLA Reheman, A ,et al."Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS and the Antioxidant Activity In Vitro".EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE 2018.3(2018):1-13.
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