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CHARACTERIZATION AND EXPRESSION ANALYSIS OF SIX CHITINASE GENES FROM THE DESERT BEETLE Microdera punctipennis IN RESPONSE TO LOW TEMPERATURE
Lu, XY (Lu, Xueying); Li, JQ (Li, Jieqiong); Liu, XN (Liu, Xiaoning); Li, XB (Li, Xiaobo); Ma, J (Ma, Ji)
2014
Source PublicationCRYOLETTERS
Volume35Issue:5Pages:438-448
Abstract

BACKGROUND: Chitinase is responsible for chitin metabolism in a wide range of organisms. However, current knowledge on insect chitinase and their possible functions in relation to low temperature stress is very limited. OBJECTIVE: Six chitinase genes from cold treated desert beetle Microdera punctipennis obtained by RNA-seq technology were characterized, and their expression patterns in different tissues and in response to cold were investigated. METHODS: Multiple sequence alignment was carried out using ClustalW1.81 and Phylogenetic trees were generated by MEGA5. The expression patterns were studied by quantitative real-time PCR. RESULTS: These genes were belong to three different chitinase groups. Almost all of them were highly expressed in midgut, and some are expressed in fat body or hindgut. Subzero-4 degrees C had stronger effect than 4 degrees C in inducing chitinase expression. CONCLUSION: The tissue specific and cold inducible expressions suggest that the chitinases may have diverse functions and play roles in insect cold adaptation.

KeywordMicrodera Punctipennis Chitinase Genes Cold Stress Tissue-specific Gene Expression
Indexed BySCI
WOS IDWOS:000345495800011
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Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/5227
Collection省部共建新疆特有药用资源利用重点实验室
Affiliation1.Xinjiang Univ, Coll Life Sci & Technol, Key Lab Xinjiang Biol Resources & Genet Engn, Urumqi, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Chem Plant Resources Arid Reg, Urumqi, Peoples R China
Recommended Citation
GB/T 7714
Lu, XY ,Li, JQ ,Liu, XN ,et al. CHARACTERIZATION AND EXPRESSION ANALYSIS OF SIX CHITINASE GENES FROM THE DESERT BEETLE Microdera punctipennis IN RESPONSE TO LOW TEMPERATURE[J]. CRYOLETTERS,2014,35(5):438-448.
APA Lu, XY ,Li, JQ ,Liu, XN ,Li, XB ,&Ma, J .(2014).CHARACTERIZATION AND EXPRESSION ANALYSIS OF SIX CHITINASE GENES FROM THE DESERT BEETLE Microdera punctipennis IN RESPONSE TO LOW TEMPERATURE.CRYOLETTERS,35(5),438-448.
MLA Lu, XY ,et al."CHARACTERIZATION AND EXPRESSION ANALYSIS OF SIX CHITINASE GENES FROM THE DESERT BEETLE Microdera punctipennis IN RESPONSE TO LOW TEMPERATURE".CRYOLETTERS 35.5(2014):438-448.
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