Development of HPLC Protocol and Simultaneous Quantification of Four Free Flavonoids from Dracocephalum heterophyllum Benth
Numonov, SR (Numonov, Sodik Rakhmonovich); Qureshi, MN (Qureshi, Muhammad Nasimullah); Aisa, HA (Aisa, Haji Akber); Aisa, HA
2015
发表期刊INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY
卷号2015期号:4
摘要

Quantification of the four flavonoids, namely, luteolin, kaempferol, diosmetin, and chrysosplenetin, has been performed for the first time in 80% ethanolic extract of Dracocephalum heterophyllum B. through HPLC coupled to UV detector after optimization of extracting solvent and chromatographic conditions. Total flavonoids quantified were 0.324 mg/ mL of the extract. HPLC analysis delivered contents of the luteolin, kaempferol, diosmetin, and chrysosplenetin as 0.08%, 0.14%, 0.28%, and 0.79% of the dried extract, respectively. LOD (%) values calculated were 0.04, 0.03, 0.03, and 0.08 and LOQ (%) values were 0.08, 0.12, 0.11, and 0.28 for luteolin, kaempferol, diosmetin, and chrysosplenetin, respectively. The recovery percentages for these flavonoids were within the acceptable range of 95% to 105%. Standard deviation and %RSD were calculated for each target analytes individually in extract for determining the reproducibility and accuracy of the method. In no case the % RSD was higher than 1 taking retention time as a factor while in the case of area under the curve maximum % RSD was noted in the case of diosmetin as 2.85. From our literature review regarding the plant species under study, it appears that these flavonoids have not been quantified before and are reported for the first time in this paper.

DOI10.1155/2015/503139
收录类别SCI
WOS记录号WOS:000354305700001
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文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/5187
专题省部共建新疆特有药用资源利用重点实验室
通讯作者Aisa, HA
作者单位1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Reg, Urumqi 830011, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China
3.Acad Sci Republ Tajikistan, State Sci Expt & Prod Org, Dushanbe 734063, Tajikistan
4.Abdul Wali Khan Univ, Dept Chem, Mardan 23200, Pakistan
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Numonov, SR ,Qureshi, MN ,Aisa, HA ,et al. Development of HPLC Protocol and Simultaneous Quantification of Four Free Flavonoids from Dracocephalum heterophyllum Benth[J]. INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY,2015,2015(4).
APA Numonov, SR ,Qureshi, MN ,Aisa, HA ,&Aisa, HA.(2015).Development of HPLC Protocol and Simultaneous Quantification of Four Free Flavonoids from Dracocephalum heterophyllum Benth.INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY,2015(4).
MLA Numonov, SR ,et al."Development of HPLC Protocol and Simultaneous Quantification of Four Free Flavonoids from Dracocephalum heterophyllum Benth".INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY 2015.4(2015).
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