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Separation of Epigallocatechin Gallate from Natural Plant Extracts Using Crowding Agents-Assisted Imprinted Polymers
Sun, X (Sun, Xuan); Zhang, C (Zhang, Chen); Huang, YP (Huang, Yan-Ping); Liu, ZS (Liu, Zhao-Sheng)
2015
Source PublicationCHROMATOGRAPHIA
Volume78Issue:15-16Pages:995-1003
Abstract

A methodology-based molecularly imprinted polymer (MIP) was proposed to separate epigallocatechin gallate (EGCG) from natural plant extracts. A molecular crowding agent was introduced to improve the affinity and recognizing ability of EGCG in the preparation of an MIP monolith. Acrylamide was used as the functional monomer, and ethylene glycol dimethacrylate was the crosslinking monomer, using a solution of polystyrene in tetrahydrofuran as a molecular crowding agent with isooctane as a coporogen. In addition, it was found that the ratio of the macromolecule agent, the amount of templates and crosslinking degree, and the composition of the mobile phase greatly affected the retention of the template and performance of molecular recognition. The imprinting factor of the resulting MIP monolith obtained was up to 9.67. The resulting MIP can be used to purify EGCG from crude tea polyphenol efficiently with mean recoveries of 87.42 %.

KeywordMonolithic Column Molecularly Imprinted Polymer Molecular Crowding Solid Phase Extraction Epigallocatechin Gallate
DOI10.1007/s10337-015-2914-y
Indexed BySCI
WOS IDWOS:000358599200005
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/5150
Collection省部共建新疆特有药用资源利用重点实验室
Affiliation1.Tianjin Med Univ, Sch Pharm, Key Lab Technol Enabling Dev Clin Therapeut & Dia, Tianjin 300070, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Peoples R China
Recommended Citation
GB/T 7714
Sun, X ,Zhang, C ,Huang, YP ,et al. Separation of Epigallocatechin Gallate from Natural Plant Extracts Using Crowding Agents-Assisted Imprinted Polymers[J]. CHROMATOGRAPHIA,2015,78(15-16):995-1003.
APA Sun, X ,Zhang, C ,Huang, YP ,&Liu, ZS .(2015).Separation of Epigallocatechin Gallate from Natural Plant Extracts Using Crowding Agents-Assisted Imprinted Polymers.CHROMATOGRAPHIA,78(15-16),995-1003.
MLA Sun, X ,et al."Separation of Epigallocatechin Gallate from Natural Plant Extracts Using Crowding Agents-Assisted Imprinted Polymers".CHROMATOGRAPHIA 78.15-16(2015):995-1003.
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