XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室
Isolation of deoxypodophyllotoxin and podophyllotoxin from Juniperus sabina by high speed counter current chromatography
Zhao, Y (Zhao, Y.); Yang, Y (Yang, Y.); Chen, Q (Chen, Q.); Kasimu, R (Kasimu, R.); Aisa, HA (Aisa, H. Akber)
2016
Source PublicationAFINIDAD
ISSN0001-9704
Volume73Issue:575Pages:237-240
Abstract

Deoxypodophyllotoxin and podophyllotoxin are known for their excellent anti-proliferative and anti-tumor activities, therefore large amount of pure compounds is urgently needed as authentic standards for various in vivo and in vitro studies. In this paper, an effective, rapid separation and purification method of deoxypodophyllotoxin and podophyllotoxin from the crude extract of Juniperus sabina was established using high speed counter current chromatography (HSCCC). HSCCC was performed with a two phase solvent system comprising of n-hexane-ethyl acetate-methanol-water (3:5:3:5, v/v) at the flow rate of 2 mL/min at the speed of 850 rpm. 34.8 mg of deoxypodophyllotoxin and 7.9 mg of podophyllotoxin were obtained from 200 mg crude sample with a purity of 96.5% and 94.4%, respectively, as determined by high performance liquid chromatography (HPLC).

KeywordJuniperus Sabina Hsccc Deoxypodophyllotoxin Podophyllotoxin
Indexed BySCI
WOS IDWOS:000388912000012
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/5121
Collection省部共建新疆特有药用资源利用重点实验室
资源化学研究室
Corresponding AuthorAisa, HA (Aisa, H. Akber)
Affiliation1.Chinese Acad Sci, Key Lab Plant Resources & Chem Arid Reg, Urumqi, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China
3.Xin Jiang Med Univ, Urumqi 830011, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Y ,Yang, Y ,Chen, Q ,et al. Isolation of deoxypodophyllotoxin and podophyllotoxin from Juniperus sabina by high speed counter current chromatography[J]. AFINIDAD,2016,73(575):237-240.
APA Zhao, Y ,Yang, Y ,Chen, Q ,Kasimu, R ,&Aisa, HA .(2016).Isolation of deoxypodophyllotoxin and podophyllotoxin from Juniperus sabina by high speed counter current chromatography.AFINIDAD,73(575),237-240.
MLA Zhao, Y ,et al."Isolation of deoxypodophyllotoxin and podophyllotoxin from Juniperus sabina by high speed counter current chromatography".AFINIDAD 73.575(2016):237-240.
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