Isolation of deoxypodophyllotoxin and podophyllotoxin from Juniperus sabina by high speed counter current chromatography
Zhao, Y (Zhao, Y.); Yang, Y (Yang, Y.); Chen, Q (Chen, Q.); Kasimu, R (Kasimu, R.); Aisa, HA (Aisa, H. Akber); Aisa, HA
2016
发表期刊AFINIDAD
卷号73期号:575页码:237-240
摘要

Deoxypodophyllotoxin and podophyllotoxin are known for their excellent anti-proliferative and anti-tumor activities, therefore large amount of pure compounds is urgently needed as authentic standards for various in vivo and in vitro studies. In this paper, an effective, rapid separation and purification method of deoxypodophyllotoxin and podophyllotoxin from the crude extract of Juniperus sabina was established using high speed counter current chromatography (HSCCC). HSCCC was performed with a two phase solvent system comprising of n-hexane-ethyl acetate-methanol-water (3:5:3:5, v/v) at the flow rate of 2 mL/min at the speed of 850 rpm. 34.8 mg of deoxypodophyllotoxin and 7.9 mg of podophyllotoxin were obtained from 200 mg crude sample with a purity of 96.5% and 94.4%, respectively, as determined by high performance liquid chromatography (HPLC).

关键词Juniperus Sabina Hsccc Deoxypodophyllotoxin Podophyllotoxin
收录类别SCI
WOS记录号WOS:000388912000012
引用统计
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/5121
专题省部共建新疆特有药用资源利用重点实验室
通讯作者Aisa, HA
作者单位1.Chinese Acad Sci, Key Lab Plant Resources & Chem Arid Reg, Urumqi, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China
3.Xin Jiang Med Univ, Urumqi 830011, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Y ,Yang, Y ,Chen, Q ,et al. Isolation of deoxypodophyllotoxin and podophyllotoxin from Juniperus sabina by high speed counter current chromatography[J]. AFINIDAD,2016,73(575):237-240.
APA Zhao, Y ,Yang, Y ,Chen, Q ,Kasimu, R ,Aisa, HA ,&Aisa, HA.(2016).Isolation of deoxypodophyllotoxin and podophyllotoxin from Juniperus sabina by high speed counter current chromatography.AFINIDAD,73(575),237-240.
MLA Zhao, Y ,et al."Isolation of deoxypodophyllotoxin and podophyllotoxin from Juniperus sabina by high speed counter current chromatography".AFINIDAD 73.575(2016):237-240.
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