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Isoliquiritigenin suppresses IL-1 beta induced apoptosis and inflammation in chondrocyte-like ATDC5 cells by inhibiting NF-kappa B and exerts chondroprotective effects on a mouse model of anterior cruciate ligament transection
Ji, BC (Ji, Baochao); Guo, WT (Guo, Wentao); Ma, HR (Ma, Hairong); Xu, BY (Xu, Boyong); Mu, WB (Mu, Wenbo); Zhang, ZD (Zhang, Zhendong); Amat, A (Amat, Abdusami); Cao, L (Cao, Li)
2017
Source PublicationINTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
ISSN1107-3756
Volume40Issue:6Pages:1709-1718
Abstract

Isoliquiritigenin (ISL), a natural flavonoid extracted from licorice, has been demonstrated to exert attenuation of the nuclear factor-kappa B (NF-kappa B) signaling pathway and anti-inflammatory activity in a wide variety of cells. In the present study, the authors first evaluated the effects of ISL on cartilage degeneration in interleukin-1 beta (IL-1 beta)-stimulated chondrocyte-like ATDC5 cells and in a mouse model of osteoarthritis (OA). The data of a cell counting kit-8 and flow cytometry assay indicated that ISL suppressed the inhibitory effect of IL-1 beta on cell viability. The mRNA and protein expression levels of cyclooxygenase-2 and matrix metalloproteinase-13 were significantly decreased, while the expression of collagen II was increased, as indicated by RT-qPCR and western blot analysis following the chondrocyte-like ATDC5 cells were co-intervened with IL-1 beta and ISL for 48 h. Also, ISL attenuated protein expressions level of pro-apoptotic Bax, cleaved-caspase-3 and cleaved-caspase-9 and promoted expression of anti-apoptotic Bcl-2. Moreover, ISL inhibited NF-kappa B p65 phosphorylation induced by IL-1 beta. In addition, ISL also increased improved the thickness of hyaline cartilage and the production of proteoglycans in the cartilage matrix in a mouse OA model. These results indicated that ISL exerted anti-inflammatory and anti-apoptotic effects on IL-1 beta-stimulated chondrocyte-like ATDC5 cells, which may be associated with the downregulation of the NF-kappa B signaling pathway. In this way, the data supported the conclusion that ISL may be a novel potential preventive agent suitable for use in OA therapy.

KeywordIsoliquiritigenin Osteoarthritis Chondrocytes Anti-apoptosis Anti-catabolic
DOI10.3892/ijmm.2017.3177
Indexed BySCI
WOS IDWOS:000418226300011
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/5079
Collection资源化学研究室
Affiliation1.Xinjiang Med Univ, Affiliated Hosp 1, Dept Orthopaed, 137 South Liyushan Rd, Urumqi 830054, Xinjiang, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830054, Xinjiang, Peoples R China
Recommended Citation
GB/T 7714
Ji, BC ,Guo, WT ,Ma, HR ,et al. Isoliquiritigenin suppresses IL-1 beta induced apoptosis and inflammation in chondrocyte-like ATDC5 cells by inhibiting NF-kappa B and exerts chondroprotective effects on a mouse model of anterior cruciate ligament transection[J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,2017,40(6):1709-1718.
APA Ji, BC .,Guo, WT .,Ma, HR .,Xu, BY .,Mu, WB .,...&Cao, L .(2017).Isoliquiritigenin suppresses IL-1 beta induced apoptosis and inflammation in chondrocyte-like ATDC5 cells by inhibiting NF-kappa B and exerts chondroprotective effects on a mouse model of anterior cruciate ligament transection.INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,40(6),1709-1718.
MLA Ji, BC ,et al."Isoliquiritigenin suppresses IL-1 beta induced apoptosis and inflammation in chondrocyte-like ATDC5 cells by inhibiting NF-kappa B and exerts chondroprotective effects on a mouse model of anterior cruciate ligament transection".INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 40.6(2017):1709-1718.
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