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薰衣草精油蒸馏残渣化学成分及其生物活性研究
尼格尔热依·亚迪卡尔
学位类型博士
导师阿吉艾克拜尔·艾萨
2017-06-03
学位授予单位中国科学院大学
学位授予地点北京
学位专业有机化学
关键词薰衣草 残渣 酚酸类化合物 Dpph 抗氧化活性
摘要

薰衣草 (Lavender),属唇形科典型的芳香植物,首载于阿维森纳著《医典》中,还被中华人民共和国卫生部《药品标准》维吾尔药分册,《维吾尔药材标准》等收载,是维吾尔族习用药材,存在于20多种维吾尔药复方制剂中。大量文献调研表明,目前薰衣草精油的提取多采取水蒸气蒸馏法,其化学成分包括芳樟醇、乙酸芳樟酯、4-松油醇、乙酸薰衣草酯等100多种,且具有镇静催眠、解痉挛、抑菌、消炎镇痛、抗氧化、抗凝血、降脂降压、抗突变、保护神经、驱虫等多方面的医疗功效;薰衣草非挥发性组分包括大量黄酮类及酚酸类等活性物质,但对其研究很少。我国新疆伊犁地区的薰衣草产量巨大,品质优良,但多以干花或精油形式生产,大量薰衣草精油提取后产生的残渣只能作为牲畜的饲料及农作物肥料进行处理,造成了很大的资源浪费。本论文以新疆伊犁地区的薰衣草 (Lavandual angustifolia Mill.) 经水蒸气蒸馏法提取精油后产生的残渣为研究对象,对其进行系统地化学成分分析及生物活性检测;该研究不仅打破了国内外对熏衣草残渣探索甚少的局面,同时变废为宝,为薰衣草植物药用价值的进一步开发再利用,提高薰衣草产品技术附加值提供理论与技术支持;同时,残渣的再利用减少了对环境的污染,对当地经济可持续发展也具有重要意义。其具体内容如下:1. 对薰衣草残渣的化学成分进行了系统地分离鉴定,对其含水乙醇提取物的乙酸乙酯与正丁醇萃取部位,运用硅胶、Sephadex LH-20、ODS、大孔吸附树脂、制备高效液相色谱等分离方法,结合Optical Rotation、MS、UV、1D NMR、2D NMR等分析方法,分离并鉴定出了60个化合物,包括肉桂酸类化合物13个,氢化肉桂酸类化合物9个,苯乳酸类化合物3个,咖啡酸类化合物2个,丹酚酸类化合物15个,黄酮类化合物5个,香豆素类化合物2个,其他酚酸类化合物6个,以及其他类型化合物5个。其中,化合物1、2、3、14、15、28、29、30、31、32、33、34、35、50、56为15个新化合物;除了化合物26、39、43、44、46及59以外,其余54个化合物均为首次从薰衣草中分离得到。2. 采用DPPH自由基清除法对各极性部位及分离纯化得到的单体化合物的抗氧化活性进行了初步的评价。实验结果表明薰衣草残渣的总提取物、乙酸乙酯及正丁醇萃取物具有一定DPPH自由基清除能力;单体化合物中丹酚酸类化合物表现出很强的DPPH自由基清除活性,其中新化合物28、29、30、32、33、34的结果优于阳性对照维生素C,其他化合物26、27、36、37、39、40、41、42、46、47、52、57、58等也具有良好的抗氧化活性。

其他摘要

Lavender, which is a typical aromatic plant of the Labiatae, was firstly recorded in "Canon of Medicine" of Avicenna, now is officially listed in 《Drug Standard of Ministry of Public Health of the People's Republic of China (Part of Uyghur Medicines)》, 《Uygur Medicine Standard》, and so on. Today, Lavender has been widely used as Traditional Uighur Medicine, which is existing in more than 20 kinds of uygur medicine compound preparations.A lot of research literatures show that, the steam distillation has been used widely for extracting essential oil from Lavender, and there are more than 100 individual components found in the Lavender oil, such as linalool, linalyl acetate, 4-terpineol and lavender acetate, which have various medical efficacy in terms of sedative hypnotic, spasmolysis, anti-bacterial, anti-inflammatory, analgesic, anti-oxidation, anti-coagulant, lipid-lowering buck, anti-mutation, nerve protection, and desinsectization. Meanwhile, there are very few researches about non-volatile part of Lavender, which is rich in active substances like flavonoids and phenolic acids. The Yili region of Xinjiang (Northwest of China) abounds with Lavender whose quality is excellent, but only produced into dried or essential oil productions mostly. Thus, a large number of the remaining material which is obtained from Lavender, after the essential oil being distillated, has only been used as livestock feed and crop fertilizer, that causes great waste of resources. Therefore, the present study mainly focused on the chemical constituents isolated from the remaining material of Lavandual angustifolia Miller after steam distillation, and their biological activities. This work not only broke the situation that the waste of Lavender had been researched very little at home and abroad, but also turned waste into treasure, and would be of great importance in further development and reuse of the medicinal value of Lavender, and in providing theoretical and technical support for enhancing the additional value of the commodity technology, and in reducing environmental pollution, and in sustainable development of the local economy.1. The systematic isolation and identification of the chemical composition from the waste of Lavandual angustifolia Miller. By using a variety of chromatographic techniques, such as silical gel, sephadex LH-20, ODS, macroporous resin, and semi-preparative HPLC, combining with the extensive analysis of Optical Rotation, MS, UV, 1D NMR, and 2D NMR, 60 compounds were isolated from ethyl acetate and n-butanol extractants of aqueous ethanol extract. There were 13 cinnamic acids, 9 hydrogenated cinnamic acids, 3 benzene lactic acids, 2 caffeic acids, 15 salvianolic acids, 5 flavonoids, 2 coumarins, 6 other phenolic acids, and 5 other types of compounds, including 15 new compounds: 1, 2, 3, 14, 15, 28, 29, 30, 31, 32, 33, 34, 35, 50, and 56. Except for 26, 39, 43, 44, 46 and 59, all other 54 compounds were isolated from Lavender for the first time.2. The anti-oxidant activitives of each polarity fraction and the isolated compounds were preliminary evaluated by DPPH radical scavenging method. The results showed that the total extractant, the ethyl acetate extractant, and the n-butanol extractant of the Lavender waste, had a certain degree of DPPH free radical scavenging abilities. Then, the salvianolic acids isolated showed strong DPPH free radical scavenging abilities. Especially, the IC50 values of new compounds 28, 29, 30, 32, 33, 34 were superior to that of positive control (Vitamin C). Other compounds such as 26, 27, 36, 37, 39, 40, 41, 42, 46, 47, 52, 57, and 58, were also revealing good anti-oxidant activities.

文献类型学位论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/4972
专题资源化学研究室
作者单位中国科学院新疆理化技术研究所
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尼格尔热依·亚迪卡尔. 薰衣草精油蒸馏残渣化学成分及其生物活性研究[D]. 北京. 中国科学院大学,2017.
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