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Effect of Chlorogenic Acid on Melanogenesis of B16 Melanoma Cells
Li, HR (Li, Hao-Rong)1; Habasi, M (Habasi, Maidina)1; Xie, LZ (Xie, Lian-Zhen)1; Aisa, HA (Aisa, Haji Akber)1; Aisa, HA
2014
Source PublicationMOLECULES
Volume19Issue:9Pages:12940-12948
AbstractChlorogenic acid (CGA), the ester formed between caffeic acid and L-quinic acid, is a widespread phenolic compound. It is part of the human diet, found in foods such as coffee, apples, pears, etc. CGA is also was widely used in cosmetics, but the effects of CGA on melanogenesis are unknown. In this study, we analyzed the effects of CGA on cell proliferation, melanin content and tyrosinase of B16 murine melanoma cells. Additionally, the enzymatic reactions of CGA in B16 melanoma cells lytic solution were detected by UV spectrophotometry. Results showed CGA at 30 and 60 mu M significantly suppresses cell proliferation. 8-MOP at 100 mu M significantly promotes cell proliferation, but CGA can counter this. Incubated for 24 h, CGA (500 mu M) improves melanogenesis while suppressing tyrosinase activity in B16 melanoma cells or 8-methoxypsoralen (8-MOP) co-incubated B16 melanoma cells. After 12 h, B16 melanoma cell treatment with CGA leads to an increase in melanin accumulation, however, after 48 h there is a decrease in melanin production which correlates broadly with a decrease in tyrosinase activity. CGA incubated with lytic solution 24 h turned brown at 37 degrees C. The formation of new products (with a maximum absorption at 295 nm) is associated with reduction of CGA (maximum absorption at 326 nm). Therefore, CGA has its two sidesroles in melanogenesis of B16 melanoma cells. CGA is a likely a substrate of melanin, but the metabolic product(s) of CGA may suppress melanogenesis in B16 melanoma cells by inhibiting tyrosinase activity.
KeywordChlorogenic Acid Melanogenesis Tyrosinase Cell Lytic Solution Browning Reaction
DOI10.3390/molecules190912940
Indexed BySCI
WOS IDWOS:000343093100004
Citation statistics
Cited Times:33[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/4907
Collection省部共建新疆特有药用资源利用重点实验室
Corresponding AuthorAisa, HA
Affiliation1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Zone, Urumqi 830011, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Peoples R China
3.Univ Chinese Acad Sci, Beijing 1008611, Peoples R China
Recommended Citation
GB/T 7714
Li, HR ,Habasi, M ,Xie, LZ ,et al. Effect of Chlorogenic Acid on Melanogenesis of B16 Melanoma Cells[J]. MOLECULES,2014,19(9):12940-12948.
APA Li, HR ,Habasi, M ,Xie, LZ ,Aisa, HA ,&Aisa, HA.(2014).Effect of Chlorogenic Acid on Melanogenesis of B16 Melanoma Cells.MOLECULES,19(9),12940-12948.
MLA Li, HR ,et al."Effect of Chlorogenic Acid on Melanogenesis of B16 Melanoma Cells".MOLECULES 19.9(2014):12940-12948.
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