Evaluation of DNA barcode candidates for the discrimination of Artemisia L.
Liu, Geyu; Ning, Huixia; Ayidaerhan, Nurbolati; Aisa, Haji Akber
2016
发表期刊Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis
期号9
摘要Because of the very similar morphologies and wide diversity of Artemisia L. varieties, they are difficult to identify, and there have been many arguments about the systematic classification Artemisia L., especially concerning the division of species. DNA barcode technology is used to rapidly identify species based on standard short DNA sequences. To evaluate seven candidate DNA barcodes (ITS, ITS2, psbA-trnH, rbcL, matK, rpoB, and rpoC1) regarding their ability to identify closely related species of the Artemisia genus in Xinjiang. The corresponding PCR amplification efficiency, detectable genetic divergence, identification efficiency and phylogenetic tree were assessed. We found that the internal transcribed spacer (ITS) region exhibited the highest interspecific divergence, which was significantly higher than the observed intraspecific variation and showed the highest identification efficiency, followed by ITS2, psbA-trnH and, finally, rpoB. matK, rbcL, and rpoC1 performed poorly in this evaluation. ITS, ITS2, and psbA-trnH were able to perfectly identify the tested species Artemisia annua, A. absinthium, A. rupestris, A. tonurnefortiana, A. austriaca, A. dracunculus, A. vulgaris, and A. macrocephala. Therefore, we propose the ITS, ITS2, and psbA-trnH regions as promising DNA barcodes for the closely related species of Artemisia L. in Xinjiang.
关键词Artemisia Dna Barcoding Identification Variation
DOI10.1080/24701394.2016.1219729
收录类别SCI
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文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/4839
专题省部共建新疆特有药用资源利用重点实验室
作者单位a Key Laboratory of Plant Resources and Chemistry in Arid Zone , Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi , Xinjiang , PR China.
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Liu, Geyu,Ning, Huixia,Ayidaerhan, Nurbolati,et al. Evaluation of DNA barcode candidates for the discrimination of Artemisia L.[J]. Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis,2016(9).
APA Liu, Geyu,Ning, Huixia,Ayidaerhan, Nurbolati,&Aisa, Haji Akber.(2016).Evaluation of DNA barcode candidates for the discrimination of Artemisia L..Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis(9).
MLA Liu, Geyu,et al."Evaluation of DNA barcode candidates for the discrimination of Artemisia L.".Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis .9(2016).
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