XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室
Evaluation of DNA barcode candidates for the discrimination of Artemisia L.
Liu, Geyu; Ning, Huixia; Ayidaerhan, Nurbolati; Aisa, Haji Akber
2016
Source PublicationMITOCHONDRIAL DNA PART A
ISSN2470-1394
Volume28Issue:6Pages:956-964
Abstract

Because of the very similar morphologies and wide diversity of Artemisia L. varieties, they are difficult to identify, and there have been many arguments about the systematic classification Artemisia L., especially concerning the division of species. DNA barcode technology is used to rapidly identify species based on standard short DNA sequences. To evaluate seven candidate DNA barcodes (ITS, ITS2, psbA-trnH, rbcL, matK, rpoB, and rpoC1) regarding their ability to identify closely related species of the Artemisia genus in Xinjiang. The corresponding PCR amplification efficiency, detectable genetic divergence, identification efficiency and phylogenetic tree were assessed. We found that the internal transcribed spacer (ITS) region exhibited the highest interspecific divergence, which was significantly higher than the observed intraspecific variation and showed the highest identification efficiency, followed by ITS2, psbA-trnH and, finally, rpoB. matK, rbcL, and rpoC1 performed poorly in this evaluation. ITS, ITS2, and psbA-trnH were able to perfectly identify the tested species Artemisia annua, A. absinthium, A. rupestris, A. tonurnefortiana, A. austriaca, A. dracunculus, A. vulgaris, and A. macrocephala. Therefore, we propose the ITS, ITS2, and psbA-trnH regions as promising DNA barcodes for the closely related species of Artemisia L. in Xinjiang.

KeywordArtemisia Dna Barcoding Identification Variation
DOI10.1080/24701394.2016.1219729
Indexed BySCI
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Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/4839
Collection省部共建新疆特有药用资源利用重点实验室
Corresponding AuthorAisa, Haji Akber
Affiliation1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Zone, Urumqi, Xinjiang, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi, Peoples R China
Recommended Citation
GB/T 7714
Liu, Geyu,Ning, Huixia,Ayidaerhan, Nurbolati,et al. Evaluation of DNA barcode candidates for the discrimination of Artemisia L.[J]. MITOCHONDRIAL DNA PART A,2016,28(6):956-964.
APA Liu, Geyu,Ning, Huixia,Ayidaerhan, Nurbolati,&Aisa, Haji Akber.(2016).Evaluation of DNA barcode candidates for the discrimination of Artemisia L..MITOCHONDRIAL DNA PART A,28(6),956-964.
MLA Liu, Geyu,et al."Evaluation of DNA barcode candidates for the discrimination of Artemisia L.".MITOCHONDRIAL DNA PART A 28.6(2016):956-964.
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