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"Two-dimensional" molecularly imprinted solid-phase extraction coupled with crystallization and high performance liquid chromatography for fast semi-preparative purification of tannins from pomegranate husk extract
Sun, GY (Sun, Guangying); Liu, YF (Liu, Yanfang); Ahat, HJ (Ahat, Hasanjan); Shen, A (Shen, Aijin); Liang, XM (Liang, Xinmiao); Xue, XY (Xue, Xingya); Luo, YQ (Luo, Yuqin); Yang, P (Yang, Pan); Liu, ZS (Liu, Zhao-Sheng); Aisa, HA (Aisa, Haji Akber)
2017
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1505Issue:7Pages:35-42
Abstract

In this study, "two dimensional" molecularly imprinted solid-phase extraction (2D-MIP-SPE) of semi preparative grade was constructed to fast purify ellagitannins in pomegranate husk extract with the help of crystallization and reverse-phase liquid chromatgoraphy(RPLC). Ellagic acid and punicalagin imprinted polymers were synthesized in batch mode and two semi-preparative MIP-SPE columns were individually packed. After investigaing "functional complementation", 2D-MIP-SPE was constructed using ellagic acid MIP and punicalagin MIP-SPE as the first and second dimension, respectively. Then, pomegranate husk extract was fast divided into four fractions individually enriching in ellagic acid, granatin A, punicalagin and ellagic acid glucoside by 2D-MIP-SPE. With the aid of crystallization and RPLC, ellagic acid (13.5 mg) and punicalagin (53.4 mg) were fast obtained in 30 min. Ellagic acid glucoside was purified to the purity near 100% with a recovery of 86.1%. Granatin A (92%) was directly obtained by 2D-MIP-SPE with the recovery of 81.8%. All above indicated that 2D-MIP-SPE was highly efficient in natural product purification. The concept of "functional complementation" was expected to be a useful tool in the construction of 2D-MIP-SPE.

Keyword"two-dimensional" Molecularly Imprinted Solid-phase Extraction Functional Complementation Crystallization High-performance Liquid Chromatography Pomegranate Husk Extract
DOI10.1016/j.chroma.2017.05.033
Indexed BySCI
WOS IDWOS:000403529700003
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/4818
Collection省部共建新疆特有药用资源利用重点实验室
资源化学研究室
Affiliation1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Key Lab Chem Plant Resources Arid Reg, Urumqi 830011, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Sun, GY ,Liu, YF ,Ahat, HJ ,et al. "Two-dimensional" molecularly imprinted solid-phase extraction coupled with crystallization and high performance liquid chromatography for fast semi-preparative purification of tannins from pomegranate husk extract[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1505(7):35-42.
APA Sun, GY .,Liu, YF .,Ahat, HJ .,Shen, A .,Liang, XM .,...&Aisa, HA .(2017)."Two-dimensional" molecularly imprinted solid-phase extraction coupled with crystallization and high performance liquid chromatography for fast semi-preparative purification of tannins from pomegranate husk extract.JOURNAL OF CHROMATOGRAPHY A,1505(7),35-42.
MLA Sun, GY ,et al.""Two-dimensional" molecularly imprinted solid-phase extraction coupled with crystallization and high performance liquid chromatography for fast semi-preparative purification of tannins from pomegranate husk extract".JOURNAL OF CHROMATOGRAPHY A 1505.7(2017):35-42.
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