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Isolation of of new polyacetylenes from the roots of Eurycoma longifolia via high-speed counter-current chromatography
Wang, ML (Wang, Minglong); Zou, HJ (Zou, Huijuan); Chen, QB (Chen, Qibin); Cao, JG (Cao, Jianguo); Aisa, HA (Aisa, Haji Akber); Huang, GZ (Huang, Guozheng)
2017
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
ISSN1570-0232
Volume1055Issue:6Pages:39-44
Abstract

Eurycoma longifolia is a tropical plant of diverse applications in folk medicine, which occurs in Southeast Asia. In this study, pre-purified fraction (0.86g) of the crude extracts from the roots of E. longifolia, was subjected to preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of hexane-ethyl acetate-methanol-water (HEMWat) at a volume ratio of 5:2:5:2 (v/v). Longifolione A (1, 19 mg, purity 96.0%) and longifolione C (3, 317 mg, purity 96.2%), together with longifolione B (2, purity 77.6%) were isolated in one run. The whole mobile and stationary phase was then blown out, concentrated in vacuo, and subjected to second HSCCC purification. Using HEMWat at a volume ratio of 6:1:6:1.2 (v/v), this fraction yielded two more new polyacetylenenes, longifolione D (4, 5 mg purity 94.5%) and longifolione E (5, 33 mg purity 96.3%). All of these five compounds are new natural products and isolated from E. longifolia for the first time. The established protocol for large-scale isolation of these polyacetylenes from E. longifolia was simple, efficient, and economical.

KeywordPolyacetylene Hsccc Eurycoma Longifolia Tongkat Ali Debilisone Longifolione
DOI10.1016/j.jchromb.2017.03.038
Indexed BySCI
WOS IDWOS:000402357800006
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/4815
Collection省部共建新疆特有药用资源利用重点实验室
资源化学研究室
AffiliationShanghai Normal Univ, Coll Life & Environm Sci, Shanghai 201418, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Zone, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Peoples R China
Recommended Citation
GB/T 7714
Wang, ML ,Zou, HJ ,Chen, QB ,et al. Isolation of of new polyacetylenes from the roots of Eurycoma longifolia via high-speed counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2017,1055(6):39-44.
APA Wang, ML ,Zou, HJ ,Chen, QB ,Cao, JG ,Aisa, HA ,&Huang, GZ .(2017).Isolation of of new polyacetylenes from the roots of Eurycoma longifolia via high-speed counter-current chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1055(6),39-44.
MLA Wang, ML ,et al."Isolation of of new polyacetylenes from the roots of Eurycoma longifolia via high-speed counter-current chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1055.6(2017):39-44.
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