XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室
Preparative and Rapid Purification of Saponins from Asparagus racemosus Root by High Performance Centrifugal Partition Chromatography
Onlom, C (Onlom, Churanya); Yang, Y (Yang, Yi); Aisa, HA (Aisa, Haji A.); Woranuch, N (Woranuch, Neti); Phrompittayarat, W (Phrompittayarat, Watoo); Putalun, W (Putalun, Waraporn); Ingkaninan, K (Ingkaninan, Kornkanok)
2017
Source PublicationNATURAL PRODUCT COMMUNICATIONS
ISSN1934-578X
Volume12Issue:2Pages:241-244
Abstract

High performance centrifugal partition chromatography (HPCPC) was applied to the rapid isolation and purification of saponin glycosides in Asparagus racernosus Willd. root. A two-phase solvent system composed of CHCl3-MeOH-water (4:4:2, v/v) in descending mode was used for the separation, yielding shatavarin IX (1) and asparacoside (2) in one step. Asparanin A (3) and shatavarin V (4) were separated by repeated HPCPC fractionation using CH2Cl2-MeOH-water (4:4:2, v/v) as the solvent system, followed by either gel-filtration or TLC. Their structures were identified by NMR spectroscopy and ESI/MS. The A. racemosus extracts and 1, 2, 3 and 4 were cytotoxic towards human hepato- and prostate-carcinoma cell lines (IC50 14-37 mu M), while primary human fibroblasts were less vulnerable (IC50 22-66 mu M), i.e., every saponin glycoside showed selectivity towards carcinoma cells compared with normal fibroblasts. HPCPC has proven rapidity to separate complex mixtures of phytochemicals yielding quantities suited to biological studies.

KeywordAsparagus Racernosus Cytotoxicity High Performance Centrifugal Partition Chromatography Saponins Carcinoma Cells
Indexed BySCI
WOS IDWOS:000395229200027
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/4750
Collection省部共建新疆特有药用资源利用重点实验室
资源化学研究室
Affiliation1.Naresuan Univ, Fac Pharmaceut Sci, Dept Pharmaceut Chem & Pharmacognosy, Bioscreening Unit, Phitsanulok 65000, Thailand
2.Naresuan Univ, Ctr Excellence Innovat Chem, Phitsanulok 65000, Thailand
3.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China
4.Naresuan Univ, Fac Pharmaceut Sci, Cosmet & Nat Prod Res Unit, Phitsanulok 65000, Thailand
5.Naresuan Univ, Fac Publ Hlth, Phitsanulok 65000, Thailand
6.Khon Kaen Univ, Fac Pharmaceut Sci, Khon Kaen 40002, Thailand
Recommended Citation
GB/T 7714
Onlom, C ,Yang, Y ,Aisa, HA ,et al. Preparative and Rapid Purification of Saponins from Asparagus racemosus Root by High Performance Centrifugal Partition Chromatography[J]. NATURAL PRODUCT COMMUNICATIONS,2017,12(2):241-244.
APA Onlom, C .,Yang, Y .,Aisa, HA .,Woranuch, N .,Phrompittayarat, W .,...&Ingkaninan, K .(2017).Preparative and Rapid Purification of Saponins from Asparagus racemosus Root by High Performance Centrifugal Partition Chromatography.NATURAL PRODUCT COMMUNICATIONS,12(2),241-244.
MLA Onlom, C ,et al."Preparative and Rapid Purification of Saponins from Asparagus racemosus Root by High Performance Centrifugal Partition Chromatography".NATURAL PRODUCT COMMUNICATIONS 12.2(2017):241-244.
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