Preparative and Rapid Purification of Saponins from Asparagus racemosus Root by High Performance Centrifugal Partition Chromatography
Onlom, C (Onlom, Churanya); Yang, Y (Yang, Yi); Aisa, HA (Aisa, Haji A.); Woranuch, N (Woranuch, Neti); Phrompittayarat, W (Phrompittayarat, Watoo); Putalun, W (Putalun, Waraporn); Ingkaninan, K (Ingkaninan, Kornkanok)
2017
发表期刊NATURAL PRODUCT COMMUNICATIONS
ISSN1934-578X
卷号12期号:2页码:241-244
摘要

High performance centrifugal partition chromatography (HPCPC) was applied to the rapid isolation and purification of saponin glycosides in Asparagus racernosus Willd. root. A two-phase solvent system composed of CHCl3-MeOH-water (4:4:2, v/v) in descending mode was used for the separation, yielding shatavarin IX (1) and asparacoside (2) in one step. Asparanin A (3) and shatavarin V (4) were separated by repeated HPCPC fractionation using CH2Cl2-MeOH-water (4:4:2, v/v) as the solvent system, followed by either gel-filtration or TLC. Their structures were identified by NMR spectroscopy and ESI/MS. The A. racemosus extracts and 1, 2, 3 and 4 were cytotoxic towards human hepato- and prostate-carcinoma cell lines (IC50 14-37 mu M), while primary human fibroblasts were less vulnerable (IC50 22-66 mu M), i.e., every saponin glycoside showed selectivity towards carcinoma cells compared with normal fibroblasts. HPCPC has proven rapidity to separate complex mixtures of phytochemicals yielding quantities suited to biological studies.

关键词Asparagus Racernosus Cytotoxicity High Performance Centrifugal Partition Chromatography Saponins Carcinoma Cells
收录类别SCI
WOS记录号WOS:000395229200027
引用统计
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/4750
专题省部共建新疆特有药用资源利用重点实验室
资源化学研究室
作者单位1.Naresuan Univ, Fac Pharmaceut Sci, Dept Pharmaceut Chem & Pharmacognosy, Bioscreening Unit, Phitsanulok 65000, Thailand
2.Naresuan Univ, Ctr Excellence Innovat Chem, Phitsanulok 65000, Thailand
3.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China
4.Naresuan Univ, Fac Pharmaceut Sci, Cosmet & Nat Prod Res Unit, Phitsanulok 65000, Thailand
5.Naresuan Univ, Fac Publ Hlth, Phitsanulok 65000, Thailand
6.Khon Kaen Univ, Fac Pharmaceut Sci, Khon Kaen 40002, Thailand
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GB/T 7714
Onlom, C ,Yang, Y ,Aisa, HA ,et al. Preparative and Rapid Purification of Saponins from Asparagus racemosus Root by High Performance Centrifugal Partition Chromatography[J]. NATURAL PRODUCT COMMUNICATIONS,2017,12(2):241-244.
APA Onlom, C .,Yang, Y .,Aisa, HA .,Woranuch, N .,Phrompittayarat, W .,...&Ingkaninan, K .(2017).Preparative and Rapid Purification of Saponins from Asparagus racemosus Root by High Performance Centrifugal Partition Chromatography.NATURAL PRODUCT COMMUNICATIONS,12(2),241-244.
MLA Onlom, C ,et al."Preparative and Rapid Purification of Saponins from Asparagus racemosus Root by High Performance Centrifugal Partition Chromatography".NATURAL PRODUCT COMMUNICATIONS 12.2(2017):241-244.
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