MiR-107 suppresses proliferation of hepatoma cells through targeting HMGA2 mRNA 3 ' UTR
Wang, Y (Wang, Yuan); Chen, FQ (Chen, Fuquan); Zhao, M (Zhao, Man); Yang, Z (Yang, Zhe); Zhang, SQ (Zhang, Shuqin); Ye, LH (Ye, Lihong); Gao, HW (Gao, Hongwei); Zhang, XD (Zhang, Xiaodong)
2016
发表期刊BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷号480期号:3页码:455-460
摘要Background and aim: Aberrant expression of miR-107 is involved in the development of several human cancers. However, the role of miR-107 in hepatocellular carcinoma (HCC) is not well documented. In the present study, we aim to explore the function of miR-107 in hepatocarcinogenesis. Methods: Bioinformatics analysis was applied to predict the target genes of miR-107. Luciferase reporter gene assay was performed to verify the miR-107 binding sites in 3'-untranslated region (3'UTR) of high mobility group A2 (HMGA2) mRNA. The expression levels of mRNA and protein were examined using qRT-PCR and Western blot analysis. Functionally, MTT and EdU assays were carried out for proliferation analysis. Clinically, thirty HCC samples and their corresponding peritumor liver tissues were collected. Results: Bioinformatics analysis revealed that miR-107 might target HMGA2 mRNA 3'UTR. Luciferase reporter gene assays verified that the miR-107 binding site was located in the 3'UTR of HMGA2 mRNA. Furthermore, miR-107 could down-regulate HMGA2 at the levels of mRNA and protein in a dose dependent manner. Interestingly, miR-107 inhibited the proliferation of hepatoma cells, while antimiR-107 could promote the cell proliferation, which was blocked by the interference of HMGA2. Clinically, miR-107 was lower in HCC samples relative to peritumor liver tissues. The expression levels of miR-107 were negatively correlated with those of HMGA2 mRNA in HCC samples. Conclusion: MiR-107 suppresses the proliferation of hepatoma cells by targeting HMGA2 mRNA. Our finding provides new insights into the mechanism of hepatocarcinogenesis. (C) 2016 Elsevier Inc. All rights reserved.
关键词Mir-107 Hmga2 Cell Proliferation Hepatocellular Carcinoma
DOI10.1016/j.bbrc.2016.10.070
WOS记录号WOS:000389010900026
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被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/4720
专题省部共建新疆特有药用资源利用重点实验室
作者单位1.Nankai Univ, State Key Lab Med Chem Biol, Dept Canc Res, Coll Life Sci, 94 Weijin Rd, Tianjin 300071, Peoples R China
2.Nankai Univ, State Key Lab Med Chem Biol, Dept Biochem, Coll Life Sci, Tianjin 300071, Peoples R China
3.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Reg, Urumqi 830011, Peoples R China
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Wang, Y ,Chen, FQ ,Zhao, M ,et al. MiR-107 suppresses proliferation of hepatoma cells through targeting HMGA2 mRNA 3 ' UTR[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2016,480(3):455-460.
APA Wang, Y .,Chen, FQ .,Zhao, M .,Yang, Z .,Zhang, SQ .,...&Zhang, XD .(2016).MiR-107 suppresses proliferation of hepatoma cells through targeting HMGA2 mRNA 3 ' UTR.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,480(3),455-460.
MLA Wang, Y ,et al."MiR-107 suppresses proliferation of hepatoma cells through targeting HMGA2 mRNA 3 ' UTR".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 480.3(2016):455-460.
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