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Effect and mechanism of gallic acid on oleic acid-induced steatosis in the Chang liver cell NAFLD model
Wei, YY (Wei, Yuanyuan); Yan, D (Yan, Dong); Wang, Z (Wang, Zhong); Aisa, HA (Aisa, Haji Akber)
2016
Source PublicationINTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
ISSN1940-5901
Volume9Issue:7Pages:12669-12677
Abstract

Objective: To investigate the effect and underlying mechanism of gallic acid (GA) on oleic acid (OA)-induced triglyceride (TG) accumulation in the Chang liver cell non-alcoholic fatty liver disease (NAFLD) model. Methods: Chang liver cells were incubated with 0.2 mM OA to establish a NAFLD model. After intervention with different concentrations of GA, the treated cells were collected for determination of TG, SOD, GSH-PX, and MDA levels. Oil red O staining revealed the changes in intracellular TG accumulation after GA intervention. The levels of TNF-alpha and IL-8 in the culture medium were measured by ELISA. The levels of expression of intracellular AMPK, ACC2, and CPT-1A mRNA were evaluated by RT-PCR. The levels of phosphorylation of intracellular AMPK and ACC2 protein and the level of expression of CPT-1A protein were assessed by Western blot analysis. Results: GA intervention significantly decreased the TG level in the OA-induced NAFLD model, improved the status of steatosis, up-regulated the levels of SOD and GSH-PX, down-regulated the MDA level, and significantly reduced the levels of TNF-alpha and IL-8 in steatotic cells. In addition, the expression of AMPK and CPT-1A mRNA was up-regulated, the expression of ACC2 mRNA was down-regulated, the levels of phosphorylation of AMPK, ACC2 protein were enhanced, and the expression of CPT-1A protein was up-regulated. Conclusion: GA decreased TG accumulation in a NAFLD cell model, and was probably associated with an anti-oxidation effect. GA decreased cytokine levels and regulated the expression of enzymes related to lipid metabolism at the protein and mRNA levels.

KeywordGallic Acid Lipid Metabolism Nafld
Indexed BySCI
WOS IDWOS:000386428400026
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/4692
Collection资源化学研究室
Corresponding AuthorAisa, HA (Aisa, Haji Akber)
Affiliation1.Xinjiang Med Univ, Dept Physiol, Urumqi, Peoples R China
2.Xinjiang Med Univ, Dept Pharmacol, Urumqi, Peoples R China
3.Xinjiang Med Univ, Expt Anim Ctr, Urumqi, Peoples R China
4.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China
Recommended Citation
GB/T 7714
Wei, YY ,Yan, D ,Wang, Z ,et al. Effect and mechanism of gallic acid on oleic acid-induced steatosis in the Chang liver cell NAFLD model[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,2016,9(7):12669-12677.
APA Wei, YY ,Yan, D ,Wang, Z ,&Aisa, HA .(2016).Effect and mechanism of gallic acid on oleic acid-induced steatosis in the Chang liver cell NAFLD model.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,9(7),12669-12677.
MLA Wei, YY ,et al."Effect and mechanism of gallic acid on oleic acid-induced steatosis in the Chang liver cell NAFLD model".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 9.7(2016):12669-12677.
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