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Effect and mechanism of gallic acid on oleic acid-induced steatosis in the Chang liver cell NAFLD model
Wei, YY (Wei, Yuanyuan); Yan, D (Yan, Dong); Wang, Z (Wang, Zhong); Aisa, HA (Aisa, Haji Akber); Aisa, HA
2016
发表期刊INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
卷号9期号:7页码:12669-12677
摘要Objective: To investigate the effect and underlying mechanism of gallic acid (GA) on oleic acid (OA)-induced triglyceride (TG) accumulation in the Chang liver cell non-alcoholic fatty liver disease (NAFLD) model. Methods: Chang liver cells were incubated with 0.2 mM OA to establish a NAFLD model. After intervention with different concentrations of GA, the treated cells were collected for determination of TG, SOD, GSH-PX, and MDA levels. Oil red O staining revealed the changes in intracellular TG accumulation after GA intervention. The levels of TNF-alpha and IL-8 in the culture medium were measured by ELISA. The levels of expression of intracellular AMPK, ACC2, and CPT-1A mRNA were evaluated by RT-PCR. The levels of phosphorylation of intracellular AMPK and ACC2 protein and the level of expression of CPT-1A protein were assessed by Western blot analysis. Results: GA intervention significantly decreased the TG level in the OA-induced NAFLD model, improved the status of steatosis, up-regulated the levels of SOD and GSH-PX, down-regulated the MDA level, and significantly reduced the levels of TNF-alpha and IL-8 in steatotic cells. In addition, the expression of AMPK and CPT-1A mRNA was up-regulated, the expression of ACC2 mRNA was down-regulated, the levels of phosphorylation of AMPK, ACC2 protein were enhanced, and the expression of CPT-1A protein was up-regulated. Conclusion: GA decreased TG accumulation in a NAFLD cell model, and was probably associated with an anti-oxidation effect. GA decreased cytokine levels and regulated the expression of enzymes related to lipid metabolism at the protein and mRNA levels.
关键词Gallic Acid Lipid Metabolism Nafld
收录类别SCI
WOS记录号WOS:000386428400026
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文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/4692
专题资源化学研究室
通讯作者Aisa, HA
作者单位1.Xinjiang Med Univ, Dept Physiol, Urumqi, Peoples R China
2.Xinjiang Med Univ, Dept Pharmacol, Urumqi, Peoples R China
3.Xinjiang Med Univ, Expt Anim Ctr, Urumqi, Peoples R China
4.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China
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Wei, YY ,Yan, D ,Wang, Z ,et al. Effect and mechanism of gallic acid on oleic acid-induced steatosis in the Chang liver cell NAFLD model[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,2016,9(7):12669-12677.
APA Wei, YY ,Yan, D ,Wang, Z ,Aisa, HA ,&Aisa, HA.(2016).Effect and mechanism of gallic acid on oleic acid-induced steatosis in the Chang liver cell NAFLD model.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,9(7),12669-12677.
MLA Wei, YY ,et al."Effect and mechanism of gallic acid on oleic acid-induced steatosis in the Chang liver cell NAFLD model".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 9.7(2016):12669-12677.
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