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Electrochemical aptasensor for tetracycline using a screen-printed carbon electrode modified with an alginate film containing reduced graphene oxide and magnetite (Fe3O4) nanoparticles
Zhan, XJ (Zhan, Xuejia); Hu, GZ (Hu, Guangzhi); Wagberg, T (Wagberg, Thomas); Zhan, SS (Zhan, Shenshan); Xu, HC (Xu, Hanchu); Zhou, P (Zhou, Pei)
2016
发表期刊MICROCHIMICA ACTA
卷号183期号:2页码:723-729
摘要The authors describe a label-free electrochemical aptasensor for tetracycline (TET). The TET-binding aptamer was immobilized on a composite consisting of reduced graphene oxide, magnetite (Fe3O4) and sodium alginate, and this material was used to modify the surface of a screen-printed carbon electrode (SPCE). Cyclic voltammetry was carried out to characterize the single steps in the preparation of the modified electrode and to optimize the conditions for detection. Differential pulse voltammetry (DPV) was then used to monitor the interaction between aptamer and TET by applying the electrochemical probe thionine. Under optimal conditions, TET can be quantified by DPV in the 1 nM to 5 mu M concentration range, with a detection limit as low as 0.6 nM (at an S/N ratio of 3). The method is rapid, cost-efficient, highly sensitive and specific, and therefore is considered to be a viable platform for TET analysis in food, environmental, and clinical samples.
关键词Cyclic Voltammetry Differential Pulse Voltammetry Scanning Electron Microscopy Transmission Electron Microscopy Sodium Alginate Thionine Redox Probe
DOI10.1007/s00604-015-1718-y
收录类别SCI
WOS记录号WOS:000370176500026
引用统计
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/4685
专题环境科学与技术研究室
作者单位1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China
2.Shanghai Jiao Tong Univ, Bor S Luh Food Safety Res Ctr, Shanghai 200240, Peoples R China
3.Shanghai Jiao Tong Univ, Minist Agr, Key Lab Urban Agr South, Shanghai 200240, Peoples R China
4.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Lab Environm Sci & Technol, Urumqi 830011, Peoples R China
5.Umea Univ, Dept Phys, S-90187 Umea, Sweden
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Zhan, XJ ,Hu, GZ ,Wagberg, T ,et al. Electrochemical aptasensor for tetracycline using a screen-printed carbon electrode modified with an alginate film containing reduced graphene oxide and magnetite (Fe3O4) nanoparticles[J]. MICROCHIMICA ACTA,2016,183(2):723-729.
APA Zhan, XJ ,Hu, GZ ,Wagberg, T ,Zhan, SS ,Xu, HC ,&Zhou, P .(2016).Electrochemical aptasensor for tetracycline using a screen-printed carbon electrode modified with an alginate film containing reduced graphene oxide and magnetite (Fe3O4) nanoparticles.MICROCHIMICA ACTA,183(2),723-729.
MLA Zhan, XJ ,et al."Electrochemical aptasensor for tetracycline using a screen-printed carbon electrode modified with an alginate film containing reduced graphene oxide and magnetite (Fe3O4) nanoparticles".MICROCHIMICA ACTA 183.2(2016):723-729.
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