Cost-effective imprinting combining macromolecular crowding and a dummy template for the fast purification of punicalagin from pomegranate husk extract
Sun, GY (Sun, Guang-Ying); Wang, C (Wang, Chao); Luo, YQ (Luo, Yu-Qin); Zhao, YX (Zhao, Yong-Xin); Yang, J (Yang, Jian); Liu, ZS (Liu, Zhao-Sheng); Aisa, HA (Aisa, Haji Akber); Aisa, HA
2016
发表期刊JOURNAL OF SEPARATION SCIENCE
卷号39期号:10页码:1963-1970
摘要The combination of molecular crowding and virtual imprinting was employed to develop a cost-effective method to prepare molecularly imprinted polymers. By using linear polymer polystyrene as a macromolecular crowding agent, an imprinted polymer recognizable to punicalagin had been successfully synthesized with punicalin as the dummy template. The resulting punicalin-imprinted polymer presented a remarkable selectivity to punicalagin with an imprinting factor of 3.17 even at extremely low consumption of the template (template/monomer ratio of 1:782). In contrast, the imprinted polymer synthesized without crowding agent, did not show any imprinting effect at so low template amount. The imprinted polymers made by combination of molecular crowding and virtual imprinting can be utilized for the fast separation of punicalagin from pomegranate husk extract after optimizing the protocol of solid-phase extraction with the recovery of 85.3 +/- 1.2%.
关键词Dummy Template Macromolecular Crowding Molecular Imprinting Punicalagin Punicalin
DOI10.1002/jssc.201600165
收录类别SCI
WOS记录号WOS:000378683400019
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/4664
专题省部共建新疆特有药用资源利用重点实验室
通讯作者Aisa, HA
作者单位1.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Zone, Urumqi 830011, Xinjiang, Peoples R China
2.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Xinjiang, Peoples R China
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Sun, GY ,Wang, C ,Luo, YQ ,et al. Cost-effective imprinting combining macromolecular crowding and a dummy template for the fast purification of punicalagin from pomegranate husk extract[J]. JOURNAL OF SEPARATION SCIENCE,2016,39(10):1963-1970.
APA Sun, GY .,Wang, C .,Luo, YQ .,Zhao, YX .,Yang, J .,...&Aisa, HA.(2016).Cost-effective imprinting combining macromolecular crowding and a dummy template for the fast purification of punicalagin from pomegranate husk extract.JOURNAL OF SEPARATION SCIENCE,39(10),1963-1970.
MLA Sun, GY ,et al."Cost-effective imprinting combining macromolecular crowding and a dummy template for the fast purification of punicalagin from pomegranate husk extract".JOURNAL OF SEPARATION SCIENCE 39.10(2016):1963-1970.
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