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新疆棕色棉纤维中色素的提取及鉴定
陈艳成
学位类型硕士
导师李晓波
2011-05-30
学位授予单位中国科学院研究生院
学位授予地点北京
学位专业有机化学
关键词棕色棉纤维 原花青素 花色苷 鉴定
摘要1. 选取新疆五个棕色棉品种(系),分别为棕86、新彩棉1号、新彩棉5号、棕196和棕470,纤维色泽由深到浅依次为,棕86>新彩棉5号>棕196 >新彩棉1号>棕470,以普通白棉品种为参照,分别用50%MeCN-0.1%TFA水溶液(V乙腈: V三氟乙酸: V水=50: 0.1: 49.9)冷浸提取棉纤维色素,通过紫外光谱吸收和铁盐催化法鉴定出棕色棉和白色棉纤维提取液中均含有原花青素;利用铁盐催化反应比色法定量测定棕色棉和白棉纤维中原花青素含量,结果显示棕色棉纤维中原花青素含量明显高于白棉;除棕470外,棕色棉纤维的色泽同原花青素的含量呈明显相关性,棕色棉纤维的色泽越深,其原花青素的含量越高。此研究阐明了原花青素是棕色棉纤维色素中的一种重要组分。 2. 以深棕色新疆棕色棉品系“棕86”为材料研究天然彩色棉纤维色素组分。成熟的彩棉纤维经甲醇溶液(VMeOH: V浓HCl =99: 1)避光冷浸提取15 d,浓缩成浸膏。用适量蒸馏水溶解浸膏,用乙酸乙酯萃取后,水相上HP-20大孔树脂纯化色素。利用紫外吸收光谱和HPLC鉴定出棕色棉纤维提取液中含有花色苷。此研究表明花色苷是棕色棉纤维色素中的一种重要组成成分。 3. 以新疆棕色棉品系“新彩棉5号”为材料,避光冷浸提取,利用铁盐催化反应比色法测定原花青素,考察提取溶剂种类、酸种类、溶剂浓度、酸浓度及提取时间等因素对原花青素提取效果的影响。通过正交实验得出最佳棕色棉纤维中原花青素的提取工艺为:乙腈浓度为75%,三氟乙酸浓度为0.1%,料液比为1: 26,提取次数2次,每次提取5天。
其他摘要1. The five brown fiber cotton (Gossypium hirsutum L.) cultivars (lines) in Xinjiang, including Zong 86, Xincaimian 5, Zong 196, Xincaimian 1, Zong 470 and one white-fiber cultivar as control were studied in this research. The orders of brown fiber color strength were as follows: Zong 86 > Xincaimian 5 > Zong 196 > Xincaimian 1 > Zong 470. The pigments in both brown and white fibers were extracted by 50% MeCN (containning 0.1% TFA) at room temperature. Proanthocyanidins content in the 50% MeCN (containning 0.1% TFA) extractive solution was analysed by UV spectrum and ferric salts catalyst method. Furthermore, the proanthocyanidins concentration was determined using Ferric ions colorimetry. The results showed that the proanthocyanidins concentration in the fibers of all the five brown-colored cultivars was much higher than that in the white fiber cultivar; except for Zong 470, the color strength of colored cotton fibers is significantly correlated with the proanthocyanidins concentration. The deeper the color of brown fibers is, the more proanthocyanidins they contain. This study indicates that the procyanidins is one important component of the pigments in brown-colored cotton fibers. 2. Composition and characterization of fiber pigments in one brown fiber cotton cultivar (line) “Zong 86” was studied. The fiber pigments were extracted for 15d with methanol solution (VMeOH: VHCl= 99:1) at room temperature in the dark. The methanol extract was evaporated to dryness and the residue was dissolved in distilled water. The aqueous solution was extracted with Ethyl acetate and then the water phase was used to purify pigments by HP-20 macroporous resin separation. The pigment composition and characterization was analyzed by UV absorption spectrum and HPLC method. The results showed that the pigment extract contained several types of anthocyanins. This study indicated that the anthocyanins were one important component of the pigments in brown-colored cotton fibers. 3. The influence of the extraction solvent concentration, extraction acid concentration, solvent to material ratio and extracting time on fiber pigment extraction efficiency were investigated, using the brown fiber cotton cultivar “Xincaimian 5” as materials. Optimum extraction conditions were as follows: extracting solvent was 75% acetonitrile, extracting acid was 0.1%TFA, solid-liquid ratio was 1: 26, extracting time was 5 day without light and extracting twice. The proanthocyanidins concentration was determined using Ferric ions colorimetry in this experiment.
文献类型学位论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/4441
专题资源化学研究室
作者单位中国科学院新疆理化技术研究所
推荐引用方式
GB/T 7714
陈艳成. 新疆棕色棉纤维中色素的提取及鉴定[D]. 北京. 中国科学院研究生院,2011.
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