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题名: Novel Design for Centrifugal Counter-Current Chromatography: VI. Ellipsoid Column
作者: Gu, DY (Gu, Dongyu); Yang, Y (Yang, Yi); Xin, XL (Xin, Xuelei); Aisa, HA (Aisa, Haji Akber); Ito, Y (Ito, Yoichiro)
关键词: rotary-seal-free centrifuge ; hydrostatic counter-current chromatographic system ; centrifugal counter-current chromatography ; retention of the stationary phase ; protein separation ; peak resolution ; ellipsoid column
刊名: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
发表日期: 2015
DOI: 10.1080/10826076.2014.883533
卷: 38, 期:1, 页:68-73
收录类别: SCI
资助者: China National Funds for Distinguished Young Scientists;West Light Foundation of The Chinese Academy of Sciences;Major State Basic Research Development Program
摘要: A novel ellipsoid column was designed for centrifugal counter-current chromatography. Performance of the ellipsoid column with a capacity of 3.4mL was examined with three different solvent systems composed of 1-butanol-acetic acid-water (4:1:5, v/v/v) (BAW), hexane-ethyl acetate-methanol-0.1M HCl (1:1:1:1, v/v/v/v) (HEMH), and 12.5% (w/w) PEG1000 and 12.5% (w/w) dibasic potassium phosphate in water (PEG-DPP) each with suitable test samples. In dipeptide separation with BAW system, both stationary phase retention (Sf) and peak resolution (Rs) of the ellipsoid column were much higher at 0 degrees column angle (column axis parallel to the centrifugal force) than at 90 degrees column angle (column axis perpendicular to the centrifugal force), where elution with the lower phase at a low flow rate produced the best separation yielding Rs at 2.02 with 27.8% Sf at a flow rate of 0.07mL/min. In the DNP-amino acid separation with HEMW system, the best results were obtained at a flow rate of 0.05ml/min with 31.6% Sf yielding high Rs values at 2.16 between DNP-DL-glu and DNP-beta-ala peaks and 1.81 between DNP-beta-ala and DNP-L-ala peaks. In protein separation with PEG-DPP system, lysozyme and myolobin were resolved at Rs of 1.08 at a flow rate of 0.03mL/min with 38.9% Sf. Most of those Rs values exceed those obtained from the figure-8 column under similar experimental conditions previously reported.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/4209
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: NHLBI, Lab Bioseparat Technol, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi, Peoples R China;Dalian Ocean Univ, Sch Marine Sci & Environm Engn, Dalian, Peoples R China

Recommended Citation:
Gu, DY ,Yang, Y ,Xin, XL ,et al. Novel Design for Centrifugal Counter-Current Chromatography: VI. Ellipsoid Column[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2015,38(1):68-73.
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