Isoflavones Extracted from Chickpea Cicer arietinum L. Sprouts Induce Mitochondria-Dependent Apoptosis in Human Breast Cancer Cells
Chen, H (Chen, Hua); Ma, HR (Ma, Hai-Rong); Gao, YH (Gao, Yan-Hua); Zhang, X (Zhang, Xue); Habasi, M (Habasi, Madina); Hu, R (Hu, Rui); Aisa, HA (Aisa, Haji Akber); Aisa, HA
2015
发表期刊PHYTOTHERAPY RESEARCH
ISSN0951-418X
卷号29期号:2页码:210-219
摘要

Isoflavones are important chemical components of the seeds and sprouts of chickpeas. We systematically investigated the effects of isoflavones extracted from chickpea sprouts (ICS) on the human breast cancer cell lines SKBr3 and Michigan Cancer Foundation-7 (MCF-7). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed that ICS (10-60 mu g/mL) significantly inhibited the proliferation of both cell lines in a time-dependent and dose-dependent fashion. Wright-Giemsa staining as well as annexin V-fluorescein isothiocyanate and propidium iodide (Annexin V/PI) staining showed that ICS significantly increased cytoclasis and apoptotic body formation. Quantitative Annexin V/PI assays further showed that the number of apoptotic cells increased in a dose-dependent manner following ICS treatment. Semiquantitative reverse transcription PCR showed that ICS increased the expression of the apoptosis-promoting gene Bcl-2-associated X protein and decreased the expression of the antiapoptotic gene Bcl-2. Western blot analysis showed that treatment of SKBr3 and MCF-7 cells with ICS increased the expression of caspase 7, caspase 9, P53, and P21 in a dose-dependent manner. Flow cytometry assays using the fluorescent probe 3,3-dihexyloxacarbocyanine iodide showed a dose-dependent decrease in mitochondrial membrane potential following ICS treatment. Treatment using ICS also induced a dose-dependent increase in reactive oxygen species production. This is the first study to demonstrate that ICS may be a chemopreventive or therapeutic agent against breast cancer.

关键词Cicer Arietinum l Sprouts Isoflavones Mitochondria-dependent Apoptosis Breast Cancer Cells
DOI10.1002/ptr.5241
收录类别SCI
WOS记录号WOS:000349076500005
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/4207
专题省部共建新疆特有药用资源利用重点实验室
通讯作者Aisa, HA
作者单位Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Zone, Urumqi 830011, Peoples R China;Univ Chinese Acad Sci, Beijing 100049, Peoples R China;Xinjiang Med Univ, Affiliated Hosp 1, Urumqi 830011, Peoples R China
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Chen, H ,Ma, HR ,Gao, YH ,et al. Isoflavones Extracted from Chickpea Cicer arietinum L. Sprouts Induce Mitochondria-Dependent Apoptosis in Human Breast Cancer Cells[J]. PHYTOTHERAPY RESEARCH,2015,29(2):210-219.
APA Chen, H .,Ma, HR .,Gao, YH .,Zhang, X .,Habasi, M .,...&Aisa, HA.(2015).Isoflavones Extracted from Chickpea Cicer arietinum L. Sprouts Induce Mitochondria-Dependent Apoptosis in Human Breast Cancer Cells.PHYTOTHERAPY RESEARCH,29(2),210-219.
MLA Chen, H ,et al."Isoflavones Extracted from Chickpea Cicer arietinum L. Sprouts Induce Mitochondria-Dependent Apoptosis in Human Breast Cancer Cells".PHYTOTHERAPY RESEARCH 29.2(2015):210-219.
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