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题名: Isoflavones Extracted from Chickpea Cicer arietinum L. Sprouts Induce Mitochondria-Dependent Apoptosis in Human Breast Cancer Cells
作者: Chen, H (Chen, Hua); Ma, HR (Ma, Hai-Rong); Gao, YH (Gao, Yan-Hua); Zhang, X (Zhang, Xue); Habasi, M (Habasi, Madina); Hu, R (Hu, Rui); Aisa, HA (Aisa, Haji Akber)
通讯作者: Aisa, HA
关键词: Cicer arietinum L ; sprouts ; isoflavones ; mitochondria-dependent apoptosis ; breast cancer cells
刊名: PHYTOTHERAPY RESEARCH
发表日期: 2015
DOI: 10.1002/ptr.5241
卷: 29, 期:2, 页:210-219
收录类别: SCI
资助者: National Natural Science Foundation of China;Joint Funds of the National Natural Science Foundation of China
摘要: Isoflavones are important chemical components of the seeds and sprouts of chickpeas. We systematically investigated the effects of isoflavones extracted from chickpea sprouts (ICS) on the human breast cancer cell lines SKBr3 and Michigan Cancer Foundation-7 (MCF-7). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays showed that ICS (10-60 mu g/mL) significantly inhibited the proliferation of both cell lines in a time-dependent and dose-dependent fashion. Wright-Giemsa staining as well as annexin V-fluorescein isothiocyanate and propidium iodide (Annexin V/PI) staining showed that ICS significantly increased cytoclasis and apoptotic body formation. Quantitative Annexin V/PI assays further showed that the number of apoptotic cells increased in a dose-dependent manner following ICS treatment. Semiquantitative reverse transcription PCR showed that ICS increased the expression of the apoptosis-promoting gene Bcl-2-associated X protein and decreased the expression of the antiapoptotic gene Bcl-2. Western blot analysis showed that treatment of SKBr3 and MCF-7 cells with ICS increased the expression of caspase 7, caspase 9, P53, and P21 in a dose-dependent manner. Flow cytometry assays using the fluorescent probe 3,3-dihexyloxacarbocyanine iodide showed a dose-dependent decrease in mitochondrial membrane potential following ICS treatment. Treatment using ICS also induced a dose-dependent increase in reactive oxygen species production. This is the first study to demonstrate that ICS may be a chemopreventive or therapeutic agent against breast cancer.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/4207
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Zone, Urumqi 830011, Peoples R China;Univ Chinese Acad Sci, Beijing 100049, Peoples R China;Xinjiang Med Univ, Affiliated Hosp 1, Urumqi 830011, Peoples R China

Recommended Citation:
Chen, H ,Ma, HR ,Gao, YH ,et al. Isoflavones Extracted from Chickpea Cicer arietinum L. Sprouts Induce Mitochondria-Dependent Apoptosis in Human Breast Cancer Cells[J]. PHYTOTHERAPY RESEARCH,2015,29(2):210-219.
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文件名: Isoflavones Extracted from Chickpea Cicer arietinum L. Sprouts Induce Mitochondria-Dependent Apoptosis in Human Breast Cancer Cells .pdf
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