The Volatile Oil of Nardostachyos Radix et Rhizoma Induces Endothelial Nitric Oxide Synthase Activity in HUVEC Cells
Maiwulanjiang, M (Maiwulanjiang, Maitinuer); Bi, CWC (Bi, Cathy W. C.); Lee, PSC (Lee, Pinky S. C.); Xin, G (Xin, Guizhong); Miernisha, A (Miernisha, Abudureyimu); Lau, KM (Lau, Kei M.); Xiong, A (Xiong, Aizhen); Li, N (Li, Ning); Dong, TTX (Dong, Tina T. X.); Aisa, HA (Aisa, Haji A.)
2015
发表期刊PLOS ONE
ISSN1932-6203
卷号10期号:2
摘要Nardostahyos Radix et Rhizoma (NRR; the root and rhizome of Nardostachys jatamansi DC.) is a widely used medicinal herb. Historically, NRR is being used for the treatment of cardiovascular and neurological diseases. To search for active ingredients of NRR, we investigated the vascular benefit of NRR volatile oil in (i) the vasodilation in rat aorta ring, and (ii) the release of nitric oxide (NO) and the phosphorylation of endothelial NO synthase (eNOS) in cultured human umbilical vein endothelial cells (HUVECs). By measuring the fluorescence signal in cultures, application of NRR volatile oil resulted in a rapid activation of NO release as well as the phosphorylation of eNOS: both inductions were markedly reduced by L-NAME. In parallel, the phosphorylation level of Akt kinase was markedly increased by the oil treatment, which was partially attenuated by PI3K/Akt inhibitor LY294002. This inhibitor also blocked the NRR-induced NO production and eNOS phosphorylation. In HUVECs, application of NRR volatile oil elevated the intracellular Ca2+ level, and BAPTA-AM, a Ca2+ chelator, reduced the Ca2+ surge: the blockage were also applied to NRR-induced eNOS phosphorylation and NO production. These findings suggested the volatile oil of NRR was the major ingredient in triggering the vascular dilatation, and which was mediated via the NO production.
关键词Phosphorylation Injury Akt Identification Activation Jatamansi Pathway System Oxygen Enos
资助者Hong Kong Research Grants Council Theme-based Research Scheme;GRF ;Hong Kong Jockey Club Charities Trust;Foundation of The Awareness of Nature ; Hong Kong Research Grants Council Theme-based Research Scheme;GRF ;Hong Kong Jockey Club Charities Trust;Foundation of The Awareness of Nature
DOI10.1371/journal.pone.0116761
收录类别SCI
资助者Hong Kong Research Grants Council Theme-based Research Scheme;GRF ;Hong Kong Jockey Club Charities Trust;Foundation of The Awareness of Nature ; Hong Kong Research Grants Council Theme-based Research Scheme;GRF ;Hong Kong Jockey Club Charities Trust;Foundation of The Awareness of Nature
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文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/4205
专题省部共建新疆特有药用资源利用重点实验室
作者单位Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Peoples R China;Hong Kong Univ Sci & Technol, Ctr Chinese Med, Hong Kong, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi, Peoples R China
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Maiwulanjiang, M ,Bi, CWC ,Lee, PSC ,et al. The Volatile Oil of Nardostachyos Radix et Rhizoma Induces Endothelial Nitric Oxide Synthase Activity in HUVEC Cells[J]. PLOS ONE,2015,10(2).
APA Maiwulanjiang, M .,Bi, CWC .,Lee, PSC .,Xin, G .,Miernisha, A .,...&Aisa, HA .(2015).The Volatile Oil of Nardostachyos Radix et Rhizoma Induces Endothelial Nitric Oxide Synthase Activity in HUVEC Cells.PLOS ONE,10(2).
MLA Maiwulanjiang, M ,et al."The Volatile Oil of Nardostachyos Radix et Rhizoma Induces Endothelial Nitric Oxide Synthase Activity in HUVEC Cells".PLOS ONE 10.2(2015).
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