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Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, Lavandula angustifolia 'Munstead'
Wang, Xiaojun; Jin, Liang; Li, Mei; Mao, Minan; Zhao, Haiqing; Xu, Yaozu
2007
发表期刊JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY
ISSN1462-0316
卷号82期号:5页码:781-785
摘要An efficient protocol is outlined for the establishment of proliferating cell cluster cultures using a bioreactor, and subsequent regeneration of the aromatic plant, Lavandula angustifolia 'Munstead'. Red calli were obtained by culturing leaflet explants on basal MS medium supplemented with 0.23 mu M 2,4-dichlorophenoxyacetic (2,4-D) and 2.22 mu M 6-benzylaminopurine (BA), and subsequent sub-culturing. Calli were then transferred to basal MS liquid medium containing 0.23 mu M 2,4-D and 2.22 mu M BA to initiate suspension cultures. Cell clusters were obtained by sieving the initial suspensions and sub-culturing them in basal MS liquid medium supplemented with 0.23 mu M 2,4-D plus 2.22 mu M BA and 1.89 mu M abscisic acid, by means of shake culture, or bioreactor culture. After 20 d of culture, cell clusters were transferred to basal MS medium containing 1.14 mu M indole-3-acetic acid and produced a high number of regenerated plants, which were successfully transferred to soil.
收录类别SCI
WOS记录号WOS:000250489300017
引用统计
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/4118
专题资源化学研究室
作者单位Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China;Chinese Acad Sci, Grad Sch, Beijing 100830, Peoples R China;Zhejiang Univ, Inst Nucl Agr Sci, Hangzhou 310029, Peoples R China
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Wang, Xiaojun,Jin, Liang,Li, Mei,et al. Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, Lavandula angustifolia 'Munstead'[J]. JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY,2007,82(5):781-785.
APA Wang, Xiaojun,Jin, Liang,Li, Mei,Mao, Minan,Zhao, Haiqing,&Xu, Yaozu.(2007).Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, Lavandula angustifolia 'Munstead'.JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY,82(5),781-785.
MLA Wang, Xiaojun,et al."Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, Lavandula angustifolia 'Munstead'".JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY 82.5(2007):781-785.
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