Preparative high-speed counter-current chromatography (HSCCC) combined with conventional column chromatography (CC) has been used for isolation and purification of methyllycaconitine from Delphinium pseudocyanthum. n-Hexane-ethyl acetate-methanol-water, 1:1:1:2 (v/v), was used as the solvent system for HSCCC. Separation of methyllycaconitine from an HSCCC fraction was successfully achieved by CC on silica gel using chloroform-methanol, 7:1 (v/v), as mobile phase. A total of 113.45 mg methyllycaconitine of purity > 95% was obtained from 1.044 g extract of D. pseudocyanthum. Its structure was identified by MS and NMR.
Chinese Acad Sci, Xinjiang Key Lab Plant Resource & Natr Products C, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Peoples R China; Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China;Beijing Inst New Technol Applicat, Beijing 100049, Peoples R China
Gu, DongYu,Yang, Yi,Zhong, Jie,et al. High-speed counter-current chromatography combined with column chromatography for isolation of methyllycaconitine from Delphinium pseudocyanthum[J]. CHROMATOGRAPHIA,2007,66(11-12):949-951.