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题名: Efficient production of transgenic goat (Capra hircus) embryos using dual markers
作者: Wang, Liang; Liu, Tingting; Peng, Tao; Zhang, Dajiang; Wang, Hanqing; Cao, Xu; Li, Weihua; Wang, Anjiang; Zhu, Hai
关键词: fibroblasts ; neomycin resistance gene ; green fluorescent protein ; nuclear transfer ; embryos ; goats
刊名: SMALL RUMINANT RESEARCH
发表日期: 2008
DOI: 10.1016/i.smallrumres.2007.05.007
卷: 75, 期:1, 页:99-104
收录类别: SCI
摘要: The objective of this study was to develop an effective method to screen transgenic donor cells and improve the production efficiency of transgenic embryos. In the study, goat (Capra ibex) skin fibroblasts were co-transfected with two expression constructs, one containing the human alpha 1 (I) procollagen cDNA fragment (4.3 kb) and the neomycin resistance (Neo(r)) gene, and the second containing the green fluorescent protein (GFP) gene. The Neo(r) and GFP genes were used as markers for the screening of donor cells. Results revealed a significant difference (P < 0.01) in the production rate of positive transgenic blastocysts (44.6% versus 82.8%) between donor fibroblasts selected using antibiotic 6418 alone (Treatment A) and those from donor fibroblasts selected using antibiotic 6418 and GFP (Treatment B), respectively. No significant differences were found between Treatments A and B in the total somatic cell nuclear transfer (SCNT) embryo cleavage rates (71.1% versus 65.9%) and total SCNT blastocyst formation rates (18.2% versus 15.7%). The research results indicate the Neo(r) and GFP genes located at different constructs can be effectively combined by co-transfection to screen transgenic donor fibroblasts, and increase the yield of transgenic SCNT embryos carrying human alpha 1 (I) procollagen cDNA in goats.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/4110
Appears in Collections:资源化学研究室_期刊论文

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作者单位: Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Province, Lanzhou 730000, Peoples R China;Xinjiang Goldcattle Bio Inc, Urunqi 830026, Xinjiang, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Xinjiang, Peoples R China;Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Wang, Liang,Liu, Tingting,Peng, Tao,et al. Efficient production of transgenic goat (Capra hircus) embryos using dual markers[J]. SMALL RUMINANT RESEARCH,2008,75(1):99-104.
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