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Efficient production of transgenic goat (Capra hircus) embryos using dual markers
Wang, Liang; Liu, Tingting; Peng, Tao; Zhang, Dajiang; Wang, Hanqing; Cao, Xu; Li, Weihua; Wang, Anjiang; Zhu, Hai
2008
Source PublicationSMALL RUMINANT RESEARCH
ISSN0921-4488
Volume75Issue:1Pages:99-104
AbstractThe objective of this study was to develop an effective method to screen transgenic donor cells and improve the production efficiency of transgenic embryos. In the study, goat (Capra ibex) skin fibroblasts were co-transfected with two expression constructs, one containing the human alpha 1 (I) procollagen cDNA fragment (4.3 kb) and the neomycin resistance (Neo(r)) gene, and the second containing the green fluorescent protein (GFP) gene. The Neo(r) and GFP genes were used as markers for the screening of donor cells. Results revealed a significant difference (P < 0.01) in the production rate of positive transgenic blastocysts (44.6% versus 82.8%) between donor fibroblasts selected using antibiotic 6418 alone (Treatment A) and those from donor fibroblasts selected using antibiotic 6418 and GFP (Treatment B), respectively. No significant differences were found between Treatments A and B in the total somatic cell nuclear transfer (SCNT) embryo cleavage rates (71.1% versus 65.9%) and total SCNT blastocyst formation rates (18.2% versus 15.7%). The research results indicate the Neo(r) and GFP genes located at different constructs can be effectively combined by co-transfection to screen transgenic donor fibroblasts, and increase the yield of transgenic SCNT embryos carrying human alpha 1 (I) procollagen cDNA in goats.
KeywordFibroblasts Neomycin Resistance Gene Green Fluorescent Protein Nuclear Transfer Embryos Goats
DOI10.1016/i.smallrumres.2007.05.007
Indexed BySCI
WOS IDWOS:000253064800013
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Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/4110
Collection资源化学研究室
AffiliationChinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Province, Lanzhou 730000, Peoples R China;Xinjiang Goldcattle Bio Inc, Urunqi 830026, Xinjiang, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Urumqi 830011, Xinjiang, Peoples R China;Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Wang, Liang,Liu, Tingting,Peng, Tao,et al. Efficient production of transgenic goat (Capra hircus) embryos using dual markers[J]. SMALL RUMINANT RESEARCH,2008,75(1):99-104.
APA Wang, Liang.,Liu, Tingting.,Peng, Tao.,Zhang, Dajiang.,Wang, Hanqing.,...&Zhu, Hai.(2008).Efficient production of transgenic goat (Capra hircus) embryos using dual markers.SMALL RUMINANT RESEARCH,75(1),99-104.
MLA Wang, Liang,et al."Efficient production of transgenic goat (Capra hircus) embryos using dual markers".SMALL RUMINANT RESEARCH 75.1(2008):99-104.
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