A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers
IntroductionRosa rugosa flowers used as herbal medicine possess many activities. A fraction extracted by ethyl acetate exhibited strong inhibitive activity against protein tyrosine phosphatase 1B (PTP1B) in vitro. ObjectiveEstablish an efficient method of LC coupled to quadrupole time-of-flight (QTOF) with tandem MS/MS to investigate the compositions in the active fraction. MethodsChemical compositions were separated and investigated by LC/QTOF-MS/MS in negative electrospray ionisation (ESI) mode at different collision energy (CE) values. The maximal structural information was obtained for the identification of components. ResultsA total of 75 compounds including tannins, their related compounds and flavonoids were identified or partially characterised according to accurate mass and the characteristic fragments at low and high CE. Meanwhile, the fragmentation pathways of gallotannins and ellagitannins (hexahydroxydiphenoyl group and lactonised valoneoyl group) were studied and proposed and were used to trace tannins in crude extracts. ConclusionThe results suggest that this fraction is a source of PTP1B inhibitory activity with a potential for treating diabetes. Copyright (c) 2013 John Wiley & Sons, Ltd. As a traditional herbal medicine, Rosa rugosa flowers possess many activities. In this study, a LC-MS method was established for investigation of chemical compositions in a fraction with the PTP 1B inhibitory activity from Rosa rugosa. And 75 chemical compositions in this fraction were identified or tentatively characterized. The fragmentation pathways of gallotannins and ellagitannins were proposed. Knowing the phytochemical profiles of PTP 1B inhibitory activity fraction of this plant gives insights to its potential application to promote health.
A LCQTOF-MSMS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers.pdf（1013KB）
Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China
Recommended Citation:
Gu, Dongyu,Yang, Yi,Bakri, Mahinur,et al. A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers[J]. PHYTOCHEMICAL ANALYSIS,2013,24(6):661-670.
A LCQTOF-MSMS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers.pdf
