A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers
Gu, Dongyu; Yang, Yi; Bakri, Mahinur; Chen, Qibin; Xin, Xuelei; Aisa, Haji Akber; Aisa, HA
2013
发表期刊PHYTOCHEMICAL ANALYSIS
ISSN0958-0344
卷号24期号:6页码:661-670
摘要

IntroductionRosa rugosa flowers used as herbal medicine possess many activities. A fraction extracted by ethyl acetate exhibited strong inhibitive activity against protein tyrosine phosphatase 1B (PTP1B) in vitro.

ObjectiveEstablish an efficient method of LC coupled to quadrupole time-of-flight (QTOF) with tandem MS/MS to investigate the compositions in the active fraction.

MethodsChemical compositions were separated and investigated by LC/QTOF-MS/MS in negative electrospray ionisation (ESI) mode at different collision energy (CE) values. The maximal structural information was obtained for the identification of components.

ResultsA total of 75 compounds including tannins, their related compounds and flavonoids were identified or partially characterised according to accurate mass and the characteristic fragments at low and high CE. Meanwhile, the fragmentation pathways of gallotannins and ellagitannins (hexahydroxydiphenoyl group and lactonised valoneoyl group) were studied and proposed and were used to trace tannins in crude extracts.

ConclusionThe results suggest that this fraction is a source of PTP1B inhibitory activity with a potential for treating diabetes. Copyright (c) 2013 John Wiley & Sons, Ltd.

As a traditional herbal medicine, Rosa rugosa flowers possess many activities. In this study, a LC-MS method was established for investigation of chemical compositions in a fraction with the PTP 1B inhibitory activity from Rosa rugosa. And 75 chemical compositions in this fraction were identified or tentatively characterized. The fragmentation pathways of gallotannins and ellagitannins were proposed. Knowing the phytochemical profiles of PTP 1B inhibitory activity fraction of this plant gives insights to its potential application to promote health.

关键词Lc/qtof-ms/ms Flavonoids Protein Tyrosine Phosphatase 1b Inhibition Tannins Rosa Rugosa Thunb Flowers
DOI10.1002/pca.2451
收录类别SCI
WOS记录号WOS:000326032100020
引用统计
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/3858
专题省部共建新疆特有药用资源利用重点实验室
通讯作者Aisa, HA
作者单位Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China
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Gu, Dongyu,Yang, Yi,Bakri, Mahinur,et al. A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers[J]. PHYTOCHEMICAL ANALYSIS,2013,24(6):661-670.
APA Gu, Dongyu.,Yang, Yi.,Bakri, Mahinur.,Chen, Qibin.,Xin, Xuelei.,...&Aisa, HA.(2013).A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers.PHYTOCHEMICAL ANALYSIS,24(6),661-670.
MLA Gu, Dongyu,et al."A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers".PHYTOCHEMICAL ANALYSIS 24.6(2013):661-670.
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