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A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers
Gu, Dongyu; Yang, Yi; Bakri, Mahinur; Chen, Qibin; Xin, Xuelei; Aisa, Haji Akber
2013
Source PublicationPHYTOCHEMICAL ANALYSIS
ISSN0958-0344
Volume24Issue:6Pages:661-670
Abstract

IntroductionRosa rugosa flowers used as herbal medicine possess many activities. A fraction extracted by ethyl acetate exhibited strong inhibitive activity against protein tyrosine phosphatase 1B (PTP1B) in vitro.

ObjectiveEstablish an efficient method of LC coupled to quadrupole time-of-flight (QTOF) with tandem MS/MS to investigate the compositions in the active fraction.

MethodsChemical compositions were separated and investigated by LC/QTOF-MS/MS in negative electrospray ionisation (ESI) mode at different collision energy (CE) values. The maximal structural information was obtained for the identification of components.

ResultsA total of 75 compounds including tannins, their related compounds and flavonoids were identified or partially characterised according to accurate mass and the characteristic fragments at low and high CE. Meanwhile, the fragmentation pathways of gallotannins and ellagitannins (hexahydroxydiphenoyl group and lactonised valoneoyl group) were studied and proposed and were used to trace tannins in crude extracts.

ConclusionThe results suggest that this fraction is a source of PTP1B inhibitory activity with a potential for treating diabetes. Copyright (c) 2013 John Wiley & Sons, Ltd.

As a traditional herbal medicine, Rosa rugosa flowers possess many activities. In this study, a LC-MS method was established for investigation of chemical compositions in a fraction with the PTP 1B inhibitory activity from Rosa rugosa. And 75 chemical compositions in this fraction were identified or tentatively characterized. The fragmentation pathways of gallotannins and ellagitannins were proposed. Knowing the phytochemical profiles of PTP 1B inhibitory activity fraction of this plant gives insights to its potential application to promote health.

KeywordLc/qtof-ms/ms Flavonoids Protein Tyrosine Phosphatase 1b Inhibition Tannins Rosa Rugosa Thunb Flowers
DOI10.1002/pca.2451
Indexed BySCI
WOS IDWOS:000326032100020
Citation statistics
Cited Times:20[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/3858
Collection省部共建新疆特有药用资源利用重点实验室
资源化学研究室
Corresponding AuthorAisa, Haji Akber
AffiliationChinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China
Recommended Citation
GB/T 7714
Gu, Dongyu,Yang, Yi,Bakri, Mahinur,et al. A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers[J]. PHYTOCHEMICAL ANALYSIS,2013,24(6):661-670.
APA Gu, Dongyu,Yang, Yi,Bakri, Mahinur,Chen, Qibin,Xin, Xuelei,&Aisa, Haji Akber.(2013).A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers.PHYTOCHEMICAL ANALYSIS,24(6),661-670.
MLA Gu, Dongyu,et al."A LC/QTOF-MS/MS Application to Investigate Chemical Compositions in a Fraction with Protein Tyrosine Phosphatase 1B Inhibitory Activity from Rosa Rugosa Flowers".PHYTOCHEMICAL ANALYSIS 24.6(2013):661-670.
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