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题名: Ursolic Acid Induces Apoptosis Through Mitochondrial Intrinsic Pathway and Suppression of ERK1/2 MAPK in HeLa Cells
作者: Li, Yanhong; Lu, Xueying; Qi, Hongxue; Li, Xiaobo; Xiao, Xiangwen; Gao, Jianfeng
关键词: ursolic acid ; apoptosis ; HeLa cell ; mitogen-activated protein kinase (MAPK) ; dual-specific phosphatase (DUSP)
刊名: JOURNAL OF PHARMACOLOGICAL SCIENCES
发表日期: 2014
DOI: 10.1254/jphs.14017FP
卷: 125, 期:2, 页:202-210
收录类别: SCI
资助者: Natural Science Foundation of Xinjiang Uygur Autonomous Region;Western Action Project of Bureau of Resources and Environmental Science, Chinese Academy of Sciences, P. R. China
摘要: Ursolic acid (UA), a natural pentacyclic triterpenoid compound, has been demonstrated to induce apoptosis in various tumors. The aim of the present study was to elucidate the molecular mechanisms of UA-induced apoptosis in HeLa cells. Here, we reported that UA induced apoptosis through the mitochondrial intrinsic pathway in HeLa cells, as shown by release of cytosol cytochrome c, activation of caspase-9 and -3, reduction of Bcl-2 and Bcl-xL, and increase of Bax and Bak. UA down-regulated the phosphorylation of ERK1/2 and p38, whereas phosphorylation of INK was unchanged. The roles of ERK1/2 and p38 were further confirmed using the ERK1/2 inhibitor (U0126) and p38 inhibitor (SB203580). U0126 markedly increased UA-induced the Bax/Bcl-2 ratio, the increase of cytosol cytochrome c, and the levels of cleaved caspase-3, but SB203580 had little effects on the above characters, suggesting the ERK1/2 signaling pathway is required for apoptosis. Furthermore, UA up-regulated DUSP 1, 2, 4, 5, 6, 7, 9, and 10 mRNA expressions, which may be a clue for the role of dephosphorylation of ERK1/2 and p38. These data suggested that the apoptotic mechanism of UA treatment in HeLa cells was through the mitochondrial intrinsic pathway and closely associated with the suppression of the ERK1/2 signaling pathway.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/3777
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Chem Plant Resources Arid Reg, Urumqi 830011, Peoples R China;Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Peoples R China;Xinjian Educ Inst, Dept Phys Educ, Urumqi 830043, Peoples R China

Recommended Citation:
Li, Yanhong,Lu, Xueying,Qi, Hongxue,et al. Ursolic Acid Induces Apoptosis Through Mitochondrial Intrinsic Pathway and Suppression of ERK1/2 MAPK in HeLa Cells[J]. JOURNAL OF PHARMACOLOGICAL SCIENCES,2014,125(2):202-210.
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