The mechanism of hepatoprotective effect of sesquiterpene rich fraction from Cichorum glandulosum Boiss. et Huet on immune reaction-induced liver injury in mice
Xin, Xuelei; Yang, Weijun; Yasen, Mireguli; Zhao, Haiqing; Aisa, Haji Akber; Aisa, HA
2014
发表期刊JOURNAL OF ETHNOPHARMACOLOGY
ISSN0378-8741
卷号155期号:2页码:1068-1075
摘要Ethnopharmacological relevance: Cichorum glandulosum Boiss. et Huet is a traditional Uygur herbal medicine that has been used as a cholagogic and diuretic agent to improve liver function. However, the mechanism is not known for the liver-protective function. We investigated the antioxidant effects of plant extraction (CGE60) in vitro and in vivo, and find the mechanism of liver protection in Bacille Calmette-Guerin vaccine (BCG)+Lipopolysaccharides (LPS) induced liver injury in mice. Materials and methods: CGE60 was made, and the antioxidant activity was investigated by comparing the ability of scavenging 1,1-diphenyl-2-picrylhydrazyl (DPPH), and 2,2-azinobis(3-ehtylbenzothiazolin-6-sulfnicAcid) diammonium salt (ABTS) free radicals in vitro. Then, CGE60 was administrated in mice of liver damage model which was induced in mice using the BCG+LPS protocol. The CGE 60 extract was tested at three dosages: 50 mg/kg, 100 mg/kg, and 200 mg/kg. Product of lipid peroxidation (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX,), nitric oxide (NO), nitric oxide synthetase (NOS), hydroxyproline and alkaline phosphatase (ALP) contents were evaluated in liver to determine the CGE60 activity in the hepatic injury model. Tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and transforming growth factor-beta (TGF-beta) proteins were determined in the liver tissues using ELSIA. The signaling activities were evaluated in Western blot. Results: CGE60 exhibited strong antioxidant ability in vitro. With oral administration, CGE60 significantly increased the activity of CAT, SOD, GSH-PX, and decreased the level of NO, NO synthase, hydroxyproline, ALP and lipid peroxidation liver of in the BDG+ LPS model. CGE60 attenuated hepatic inflammation via down- regulation of TNF-alpha, IL-6 and TGF-beta. CGE60 blocked protein expression of cytochrome P450 2E1 (CYP2E1), nuclear factor kappa-B (NF-kappa B), phosphorylation of extracellular signal-regulated kinase (p-ERK1/2), and cyclooxygenase-2 (COX-2),but activated the expression of p-P38 MAPK. Conclusion: This study suggests that CGE60 possesses antioxidant activity and this activity associates with hepatoprotective effect in the mice of BCG +LPS model, and the mechanisms underlying these effects may involve antioxidant actions and anti-inflammation activities.
关键词Cichorum Glandulosum Boiss.et Huet Hepatoprotective Effect Immune Reaction-induced Liver Injury Antioxidant Antiinflammation
资助者Joint Funds of the National Natural Science Foundation of China;National Natural Science Foundation of China ; Joint Funds of the National Natural Science Foundation of China;National Natural Science Foundation of China ; Joint Funds of the National Natural Science Foundation of China;National Natural Science Foundation of China ; Joint Funds of the National Natural Science Foundation of China;National Natural Science Foundation of China
DOI10.1016/j.jep.2014.06.014
收录类别SCI
资助者Joint Funds of the National Natural Science Foundation of China;National Natural Science Foundation of China ; Joint Funds of the National Natural Science Foundation of China;National Natural Science Foundation of China ; Joint Funds of the National Natural Science Foundation of China;National Natural Science Foundation of China ; Joint Funds of the National Natural Science Foundation of China;National Natural Science Foundation of China
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/3740
专题省部共建新疆特有药用资源利用重点实验室
通讯作者Aisa, HA
作者单位Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi 830011, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Chem Plant Resources Arid Reg, Urumqi 830011, Peoples R China;Xinjiang Inst Mat Med, Urumqi 830002, Peoples R China
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Xin, Xuelei,Yang, Weijun,Yasen, Mireguli,et al. The mechanism of hepatoprotective effect of sesquiterpene rich fraction from Cichorum glandulosum Boiss. et Huet on immune reaction-induced liver injury in mice[J]. JOURNAL OF ETHNOPHARMACOLOGY,2014,155(2):1068-1075.
APA Xin, Xuelei,Yang, Weijun,Yasen, Mireguli,Zhao, Haiqing,Aisa, Haji Akber,&Aisa, HA.(2014).The mechanism of hepatoprotective effect of sesquiterpene rich fraction from Cichorum glandulosum Boiss. et Huet on immune reaction-induced liver injury in mice.JOURNAL OF ETHNOPHARMACOLOGY,155(2),1068-1075.
MLA Xin, Xuelei,et al."The mechanism of hepatoprotective effect of sesquiterpene rich fraction from Cichorum glandulosum Boiss. et Huet on immune reaction-induced liver injury in mice".JOURNAL OF ETHNOPHARMACOLOGY 155.2(2014):1068-1075.
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