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Alternative TitleCultivation of Pholiota squarrosa Mycelium
付振艳; 吴金平; 苟小清; 王晓军
Source Publication西北农业学报
Abstract为了提高生产效率、缩短翘鳞伞的生长周期,对翘鳞伞菌丝体的生长培养条件进行优化.单因素试验以及Plackett-Burman试验结果表明:翘鳞伞菌丝体生长的最适碳源为麦芽糖,最适氮源为酵母粉,最适pH为5,最适温度为23℃;影响翘鳞伞生长的主要因素为麦芽糖、酵母粉和K2 HPO4.通过最陡爬坡试验和正交试验确定翘鳞伞菌丝生长的最优培养基为:35 g/L麦芽糖+1.5 g/L酵母粉+0.5 g/L K2 HPO4+0.5 g/L KH2PO4+0.5 g/L MgSO4.在最佳培养条件下,菌丝体菌落直径由6.11 cm增加到7.41 cm.
Other AbstractThe cultivation conditions of Pholiota squarrosa mycelium were optimized in order to enhance product efficiency and shorten cultivation cycle. The one-factor experimental design and Plackett-Burman design showed that the optimum carbon source was maltose, the optimum nitrogen source was yeast, the optimum pH was 5, and that the optimum temperature was 23 ℃. What was more, the main factors influencing mycelium growth were maltose, yeast and K_2HPO_4. The optimum medium for Pholiota squarrosa mycelium growth was identified as 35 g/L maltose + 1.5 g/L yeast +0.5 g/L K_2HPO_4+0.5 g/L KH_2PO_4+0.5 g/L MgSO_4 by the experiments of the steepest ascent and orthogonal designs. On the optimum medium the colony diameter was increased from 6.11 cm to 7.41 cm.
Keyword翘鳞伞 生长条件 Plackett-burman设计 正交设计
Indexed ByCSCD
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GB/T 7714
付振艳,吴金平,苟小清,等. 翘鳞伞菌丝体生长培养研究[J]. 西北农业学报,2013,22(8):78-82.
APA 付振艳,吴金平,苟小清,&王晓军.(2013).翘鳞伞菌丝体生长培养研究.西北农业学报,22(8),78-82.
MLA 付振艳,et al."翘鳞伞菌丝体生长培养研究".西北农业学报 22.8(2013):78-82.
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