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题名: 翘鳞伞菌丝体生长培养研究
其他题名: Cultivation of Pholiota squarrosa Mycelium
作者: 付振艳; 吴金平; 苟小清; 王晓军
关键词: 翘鳞伞 ; 生长条件 ; Plackett-Burman设计 ; 正交设计
刊名: 西北农业学报
发表日期: 2013
卷: 22, 期:8, 页:78-82
收录类别: CSCD
摘要: 为了提高生产效率、缩短翘鳞伞的生长周期,对翘鳞伞菌丝体的生长培养条件进行优化.单因素试验以及Plackett-Burman试验结果表明:翘鳞伞菌丝体生长的最适碳源为麦芽糖,最适氮源为酵母粉,最适pH为5,最适温度为23℃;影响翘鳞伞生长的主要因素为麦芽糖、酵母粉和K2 HPO4.通过最陡爬坡试验和正交试验确定翘鳞伞菌丝生长的最优培养基为:35 g/L麦芽糖+1.5 g/L酵母粉+0.5 g/L K2 HPO4+0.5 g/L KH2PO4+0.5 g/L MgSO4.在最佳培养条件下,菌丝体菌落直径由6.11 cm增加到7.41 cm.
英文摘要: The cultivation conditions of Pholiota squarrosa mycelium were optimized in order to enhance product efficiency and shorten cultivation cycle. The one-factor experimental design and Plackett-Burman design showed that the optimum carbon source was maltose, the optimum nitrogen source was yeast, the optimum pH was 5, and that the optimum temperature was 23 ℃. What was more, the main factors influencing mycelium growth were maltose, yeast and K_2HPO_4. The optimum medium for Pholiota squarrosa mycelium growth was identified as 35 g/L maltose + 1.5 g/L yeast +0.5 g/L K_2HPO_4+0.5 g/L KH_2PO_4+0.5 g/L MgSO_4 by the experiments of the steepest ascent and orthogonal designs. On the optimum medium the colony diameter was increased from 6.11 cm to 7.41 cm.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/3716
Appears in Collections:资源化学研究室_期刊论文

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作者单位: 中国科学院新疆理化技术研究所

Recommended Citation:
付振艳,吴金平,苟小清,等. 翘鳞伞菌丝体生长培养研究[J]. 西北农业学报,2013,22(8):78-82.
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