中国科学院新疆理化技术研究所机构知识库
Advanced  
XJIPC OpenIR  > 资源化学研究室  > 期刊论文
题名: 小麦TaNADP-ME1基因重组植物表达载体构建及对水稻的遗传转化
其他题名: Construction of Plant Overexpression Vector of Wheat TaNADP-ME1 Gene and Genetic Transformation into Rice
作者: 付振艳; 刘峰; 苟小清; 王晓军
关键词: TaNADP-ME1 ; 转化 ; 水稻 ; TaNADP-ME1 ; Transformation ; Rice
刊名: 华北农学报
发表日期: 2014
卷: 29, 期:1, 页:25-27
收录类别: CSCD
资助者: 新疆维吾尔自治区自然基金会项目
摘要: 小麦TaNADP-ME1基因对干旱、盐、低温等非生物胁迫作出响应。利用重组技术成功构建了TaNADP-ME1的植物表达载体pCAME1,农杆菌介导法成功转化水稻品种日本晴成熟胚诱导的愈伤组织,通过潮霉素筛选获得了抗性愈伤,分化和生根后获得转化植株,PCR鉴定获得20株转基因水稻苗。结果为进一步确定TaNADP-ME1的基因功能奠定了基础。
英文摘要: The wheat TaNADP-ME1 gene was responsive to drought, salt and low temperature. In this research we successfully constructed the plant overexpression vector pCAME1 by recombinant technology, transformed the callus which was induced from the mature embryo of rice by agrobacterium-mediated method, acquired resistant callus by hygromycin and transformed plant by differentiation and regeneration on media. The 20 transgenic rice plants were acquired by PCR identification. This research laid a foundation for identification the function of TaNADP-ME1 gene.
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/3672
Appears in Collections:资源化学研究室_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
小麦TaNADP-ME1基因重组植物表达载体构建及对水稻的遗传转化.pdf(324KB)期刊论文作者接受稿开放获取View 联系获取全文

作者单位: 中国科学院新疆理化技术研究所;石河子大学农学院

Recommended Citation:
付振艳,刘峰,苟小清,等. 小麦TaNADP-ME1基因重组植物表达载体构建及对水稻的遗传转化[J]. 华北农学报,2014,29(1):25-27.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[付振艳]'s Articles
[刘峰]'s Articles
[苟小清]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[付振艳]‘s Articles
[刘峰]‘s Articles
[苟小清]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 小麦TaNADP-ME1基因重组植物表达载体构建及对水稻的遗传转化.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace