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Alternative TitleConstruction of Plant Overexpression Vector of Wheat TaNADP-ME1 Gene and Genetic Transformation into Rice
付振艳; 刘峰; 苟小清; 王晓军
Source Publication华北农学报
Other AbstractThe wheat TaNADP-ME1 gene was responsive to drought, salt and low temperature. In this research we successfully constructed the plant overexpression vector pCAME1 by recombinant technology, transformed the callus which was induced from the mature embryo of rice by agrobacterium-mediated method, acquired resistant callus by hygromycin and transformed plant by differentiation and regeneration on media. The 20 transgenic rice plants were acquired by PCR identification. This research laid a foundation for identification the function of TaNADP-ME1 gene.
KeywordTanadp-me1 转化 水稻 Tanadp-me1 Transformation Rice
Indexed ByCSCD
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Document Type期刊论文
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GB/T 7714
付振艳,刘峰,苟小清,等. 小麦TaNADP-ME1基因重组植物表达载体构建及对水稻的遗传转化[J]. 华北农学报,2014,29(1):25-27.
APA 付振艳,刘峰,苟小清,&王晓军.(2014).小麦TaNADP-ME1基因重组植物表达载体构建及对水稻的遗传转化.华北农学报,29(1),25-27.
MLA 付振艳,et al."小麦TaNADP-ME1基因重组植物表达载体构建及对水稻的遗传转化".华北农学报 29.1(2014):25-27.
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