XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室
靶向二肽基肽酶维药体外筛选模型的建立及应用
Alternative TitleA Screening Model for Dipeptidyl Peptidase Inhibitorsin vitro and Its Application in Uyghur Medicine
张晓翠; 赵海清; 窦君; 张富春; 阿吉艾克拜尔艾萨
2014
Source Publication新疆大学学报(自然科学版)
ISSN1000-2839
Volume31Issue:2Pages:222-226
Abstract

二肽基肽酶IV(dipeptidyl peptidaseIV,DPPIV)是治疗II型糖尿病的新靶点,糖依赖性胰岛素释放肽和肠促胰岛素胰高血糖素样肽-1在体内有促进胰岛β细胞分泌胰岛素,胰岛素的生物合成,β细胞的生长并抑制其凋亡和抑制血糖升高等功能.二肽基肽酶Ⅳ可快速降解糖依赖性胰岛素释放肽和肠促胰岛素胰高血糖素样肽-1,使其失去生物活性.本实验利用Caco-2细胞中所得到的二肽基肽酶IV,优化酶活筛选体系.确定了二肽基肽酶Ⅳ最适底物Gly-Pro-pNA浓度为0.064 mg/mL、反应温度37?C、反应时间30 min和竞争性抑制剂Ile-Pro-Ile浓度0.68 mmol/L,从而建立了靶向二肽基肽酶Ⅳ的快速筛选体系.应用该模型,对18种维药提取物进行了筛选.本研究结果将为维药的进一步开发和利用提供了理论依据.

Other Abstract

Dipeptidyl peptidase IV(DPP IV) is a new target for treating type II diabetes. Glucose-dependent insulin-releasing peptide, and the insulin glucagon-like peptide-1 could promote insulin secretion, biosynthesis, inhibit β-cell apoptosis, lower the blood sugar and other functions. DPP IV could degradate the level of glucose-dependent insulin-releasing peptide and glucagon-like peptide-1 to the loss of biological activity. Our experiment is to optimize the screening system for DPP IV inhibitors, based on DPP IV from Caco-2, results showed that the concentration of Gly-Pro-pNA is 0.064 mg/mL,reaction time is 30 minutes, temperature 37 ?C and the concentration of IIe-Pro-IIe 0.68 mmol/L. Therely astablishing a rapid screening system of dipeptidyl peptidase. Application of the model 18 Uyghur herbal extracts were screened and some had the inhibitory activity of DPP IV. The study will provide support for further exploration and utilization of Uyghur herbs.

Keyword二肽基肽酶iv Ii型糖尿病 维药 抑制剂 Dipeptidyl Peptidase Type Ii Diabetes Extract Of Uighur Medicine Inhibitors
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/3661
Collection省部共建新疆特有药用资源利用重点实验室
Affiliation新疆大学生命科学与技术学院;新疆生物资源基因工程国家重点实验室培育基地;中国科学院新疆理化技术研究所;干旱区植物资源化学重点实验室
Recommended Citation
GB/T 7714
张晓翠,赵海清,窦君,等. 靶向二肽基肽酶维药体外筛选模型的建立及应用[J]. 新疆大学学报(自然科学版),2014,31(2):222-226.
APA 张晓翠,赵海清,窦君,张富春,&阿吉艾克拜尔艾萨.(2014).靶向二肽基肽酶维药体外筛选模型的建立及应用.新疆大学学报(自然科学版),31(2),222-226.
MLA 张晓翠,et al."靶向二肽基肽酶维药体外筛选模型的建立及应用".新疆大学学报(自然科学版) 31.2(2014):222-226.
Files in This Item:
File Name/Size DocType Version Access License
靶向二肽基肽酶维药体外筛选模型的建立及应(392KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[张晓翠]'s Articles
[赵海清]'s Articles
[窦君]'s Articles
Baidu academic
Similar articles in Baidu academic
[张晓翠]'s Articles
[赵海清]'s Articles
[窦君]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[张晓翠]'s Articles
[赵海清]'s Articles
[窦君]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 靶向二肽基肽酶维药体外筛选模型的建立及应用.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.