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Alternative TitleStudy on Rapid Breeding of Chinese Rose
陈建雄; 陈艳瑞; 张艳敏
Source Publication园艺与种苗
Abstract[目的]为了快速繁育月季花的优良品种、保持品系的优良性状,最终实现月季花工厂化育苗。[方法]通过离体培养的方法对月季花进行组织培养基配方筛选试验。[结果]在月季组织培养快速繁育过程中,以腋芽为外殖体,在附加6-BA 2.0 mg/L+NAA 0.5~1.0 mg/L+GA 1.0 mg/L的MS培养基上,分化率为95%,并生长成为丛生苗,分化的幼芽转移到1/2 MS+NAA 0.5~1.0 mg/L或MS+6-BA 2.0 mg/L+IAA 1.0~1.5 mg/L的培养基上,诱导生根,形成了具有根、茎、叶的完整小植株,成活率达95%以上,极少数的试管苗还形成了花蕾。[结论]建立了迅速规模化生产月季花的组培方法。
Other AbstractThe aim was to fast breeding the fine varieties of Chinese rose and keep the fine character of its strain, finally realize the factory nursery of Chinese rose. [Method]In this paper, the tissue culture medium formula screening tests of Chinese rose have been carried out by using the method of in vitro culture. [Result]As a result, during the tissue culture and fast breeding process of Chinese rose, with axillary bud as outside body, the additional 6-BA 2.0 mg/L+NAA 0.5 1.0 mg/L+GA l. 0 mg/L on the MS culture medium, the bud differentiation rate of Chinese rose was 95%, and then it grew into clumps seedlings. Next, the germ of differentiation was transferred to the medium of 1/2 MS+NAA (0.5 1.0 mg/L) or MS+6-BA 2.0 mg/L + IAA l. 0-1.5 mg/L in order to be induced to grow root and form a complete small plant with roots, stems and leaves, the survival rate of them was more than 95%, and a handful of tube seedlings was also formed buds. [Conclusion]The rapidly transformation method to a large-scale production of Chinese rose was established.
Keyword月季花 组织培养 快速繁育 Rosa Chinansis Tissue Culture Rapid Propagation
Document Type期刊论文
Recommended Citation
GB/T 7714
陈建雄,陈艳瑞,张艳敏. 月季花的快速繁育研究[J]. 园艺与种苗,2014(5):32-34.
APA 陈建雄,陈艳瑞,&张艳敏.(2014).月季花的快速繁育研究.园艺与种苗(5),32-34.
MLA 陈建雄,et al."月季花的快速繁育研究".园艺与种苗 .5(2014):32-34.
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