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题名: 月季花的快速繁育研究
其他题名: Study on Rapid Breeding of Chinese Rose
作者: 陈建雄; 陈艳瑞; 张艳敏
关键词: 月季花 ; 组织培养 ; 快速繁育 ; Rosa chinansis ; Tissue culture ; Rapid propagation
刊名: 园艺与种苗
发表日期: 2014
期: 5, 页:32-34
资助者: 中国科学院“西部之光”西部博士项目
摘要: [目的]为了快速繁育月季花的优良品种、保持品系的优良性状,最终实现月季花工厂化育苗。[方法]通过离体培养的方法对月季花进行组织培养基配方筛选试验。[结果]在月季组织培养快速繁育过程中,以腋芽为外殖体,在附加6-BA 2.0 mg/L+NAA 0.5~1.0 mg/L+GA 1.0 mg/L的MS培养基上,分化率为95%,并生长成为丛生苗,分化的幼芽转移到1/2 MS+NAA 0.5~1.0 mg/L或MS+6-BA 2.0 mg/L+IAA 1.0~1.5 mg/L的培养基上,诱导生根,形成了具有根、茎、叶的完整小植株,成活率达95%以上,极少数的试管苗还形成了花蕾。[结论]建立了迅速规模化生产月季花的组培方法。
英文摘要: The aim was to fast breeding the fine varieties of Chinese rose and keep the fine character of its strain, finally realize the factory nursery of Chinese rose. [Method]In this paper, the tissue culture medium formula screening tests of Chinese rose have been carried out by using the method of in vitro culture. [Result]As a result, during the tissue culture and fast breeding process of Chinese rose, with axillary bud as outside body, the additional 6-BA 2.0 mg/L+NAA 0.5 1.0 mg/L+GA l. 0 mg/L on the MS culture medium, the bud differentiation rate of Chinese rose was 95%, and then it grew into clumps seedlings. Next, the germ of differentiation was transferred to the medium of 1/2 MS+NAA (0.5 1.0 mg/L) or MS+6-BA 2.0 mg/L + IAA l. 0-1.5 mg/L in order to be induced to grow root and form a complete small plant with roots, stems and leaves, the survival rate of them was more than 95%, and a handful of tube seedlings was also formed buds. [Conclusion]The rapidly transformation method to a large-scale production of Chinese rose was established.
内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/3660
Appears in Collections:资源化学研究室_期刊论文

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作者单位: 乌鲁木齐市种苗场;中国科学院新疆理化技术研究所;新疆乌鲁木齐林业局林业处

Recommended Citation:
陈建雄,陈艳瑞,张艳敏. 月季花的快速繁育研究[J]. 园艺与种苗,2014(5):32-34.
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