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Alternative TitleCloning and Bioinformatical Analysis of Dihydroflavonol 4-reductase Gene (DFR) from Petunia hybrida with Different Color
朱奇朗; 李晓波; 肖向文; 李雪源; 黄先忠; 郑巨云; 艾先涛
Source Publication生物技术通报


Other Abstract

In this study, through homologous gene cloning technology, three complete CDSs encoding dihydroflavonol 4-reductase(DFR), a key enzyme in the pathway of anthocyanin biosynthesis, were cloned from the corolla with white, red and blue color of petunia hybrid planted in Xinjiang respectively, and named PhDFR1, PhDFR2 and PhDFR3. Homology alignment indicated that the nucleotide similarity of the three DFRs is 97.79 %、96.59% and 97.99% with another DFRA gene from Petunia hybrida(GenBank access number:X15537). All these DFR CDSs encoded a polypeptide composed of 380 amino acid residues. The amino acid residues similarity of the three DFRs is 95.53 %、94.21% and 95.79% with DFRA protein(GenBank access number:CAA33544). DFRs of the three Petunia hybrida strains with different flower color contain a highly conserved NADP(H)-binding site and substrate specificity site which belong to NADB-Rossmann superfamily. They are stable proteins without signal peptide and are hydrophilic proteins probably located in cytoplasm. All of them have two transmembrane domains. alpha-helix and beta-sheet are primary secondary structural components of DFR. The beta-alpha-beta-alpha-beta structures forming Rossmann folding are symmetrically distributed on the whole. The phylogenyetic analysis of DFR proteins from different species revealed that three DFRs have closer relationship with the DFR from Dianthus caryophyllus than from the other plant species.

Keyword矮牵牛 二氢黄酮醇4-还原酶 同源克隆 生物信息学分析
Indexed ByCSCD
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Cited Times:3[CSCD]   [CSCD Record]
Document Type期刊论文
Affiliation中国科学院新疆理化技术研究所 干旱区植物资源化学重点实验室;新疆农业科学院经济作物研究所;石河子大学生命科学学院 农业生物技术重点实验室
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GB/T 7714
朱奇朗,李晓波,肖向文,等. 不同色彩矮牵牛DFR基因的克隆与生物信息学分析[J]. 生物技术通报,2013,9(9):68-76.
APA 朱奇朗.,李晓波.,肖向文.,李雪源.,黄先忠.,...&艾先涛.(2013).不同色彩矮牵牛DFR基因的克隆与生物信息学分析.生物技术通报,9(9),68-76.
MLA 朱奇朗,et al."不同色彩矮牵牛DFR基因的克隆与生物信息学分析".生物技术通报 9.9(2013):68-76.
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