On account of its brilliant color and characteristic bioactivity, lutein are now more and more being attached importance, and also become research hotspot of food, medicine and health products. Because lutein esters and lutein have the same efficacy, and in order to broaden application of lutein, this paper studied on the extraction, isolation, purification and stability of lutein esters and the detection method of lutein. The extraction of lutein esters was obtained by two different methods, organic solvent extraction and ultrasonic extraction respectively. The results showed that the optimum conditions of organic solvent extraction as follows: ratio of material to liquid: 1:25(g/mL), extraction temperation: 50℃, extraction time: 4h; the conditions of ultrasonic extraction were: ratio of material to liquid: 1:30(g/mL), ultrasonic power: 500w and ultrasonic time: 40min. Both output of lutein esters were comparative, the operation of organic solvent extraction were simple, but exhausted more time, was propitious to commercial scale; while the ultrasonic extraction need less time, but the manipulateive amount was small, was applied to laboratory. The optimum conditions of isolation and purification of lutein esters from marigold oil as follows: the conditions were ratio of material to liquid: 1:3(g/mL), extraction temperation: 50℃, cooling time: 3h when marigold oil was dissolved by acetone; and the conditions were ratio of material to liquid: 1:3.5(g/g), extraction temperation: 50℃, cooling time: 4h when the condensation from acetone was dissolved by n-butanol. Then washed the insoluble material with alcohol, the insoluble solids were so-called lutein esters which purity were over 85% after vacuum drying. The paper seeked a sort of detection method for lutein of marigold flowers and its oil. Compared with the AOAC method, this method was characteristic of the simple and fleet operation, less exhausted solvent, the low detection cost, the less environmental contamination and high accuracy. It was able to detected lutein of flowers, crude and essence products from marigold. At the same time, the paper also studied on the stability of compound formula between lutein esters and linolenic ethyl. Firstly it studied on the stability of lutein esters, and then studied on the stability of linolenic ethyl from lutein esters, thus afforded the reference of storage of lutein esters and was the base of its application.