Lavender is a perennial herb which is a plant belonging to the labiate. The head loaded with a lavender is among Aweisenna work called medicine canon. It is a valuable natural spice plant, mainly distributed in Atlantic islands ,Mediterranean and Yi Li in Xin Jiang of China. Lavender essential oil distilled from lavender is in a broad application because of its function of antibacterial, antispasm, soothing the nerves and etc. We sought a new method to separate the components of lavender. First, we achieved segmentation and enrichment by using supercritical extraction technology. Then, did separation and purification by utilization of a series of normal column chromatographic steps and filtration through SephadexLH-20, nine compounds including betulinic acid (Ⅰ),β-sitosterol (Ⅱ),β-daucosterol (Ⅲ), cholesterol (Ⅳ), scopoletin (Ⅴ), linalool (Ⅵ), linalyl acetate (Ⅶ), steraric acid (Ⅷ), methyl stearate (Ⅸ) were isolated from the extracts of the flowers of lavender from supercritical CO2 extraction. Compound Ⅳ is isolated from Labiate for the first time. Compound Ⅰ、Ⅴ、Ⅷ and Ⅸ is isolated from Lavender for the first time. We research its quality by mensurating acid number and ester number in the extracts of the flowers of lavender from supercritical CO2 extraction. The results showed that acid number was 10.4 and ester number was 98.25. We initial understood the basic information of lipoid in the extracts of the flowers of lavender from supercritical CO2 extraction and It lie the foundation for other quality. By methyl-esterification and methyl-transesterification in the appropriate condition and GC-MS , We known more lipoid components in the extracts of the flowers of lavender from supercritical CO2 extraction and also known more lipoid components in the lavender. It opened a new methodological window for the future study on the chemical composition of lavender.