|关键词||异常黏液质成熟剂 清除剂 制备工艺 成分分析 生物活性|
For more than 2500 years, the Traditional Uyghur Medicine (TUM)has been able ?to preserve its core value, and continuously develop due to a scientific theoretical ?system of the Uyghur Medicine Theory. Historically, the theory of Uyghur Medicine ?has played a key role in guiding Uyghur Medicine treatment and its development and ?promotion. But after the 19th century, due to various historical and other objective ?reasons, theoretical studies of Uyghur Medicine have been almost stationary. Today, ?development of Uyghur Medicine and the era demands us to deepen the research and ?innovate the theory of Uyghur Medicine in order to accommodate rapid expantion ?of human population and advance in the science and technology.? According to Traditional Uyghur Medicine, abnormal changes of the four body ?liquids such as Sapra(yellow bile), Kan(blood), Balgam(phlegm) and Sawda(black bile) ?causes various substantial lesions in the body. Pathological product of abnormal ?Balgam calming in perivascular tissue, generating silt resistance, changing the ?temperament of the organ tissue ,then eventually leads to refractory diseases such ?as stroke, Alzheimer's disease, diabetes, vitiligo, psoriasis, simple obesity, rheumatism, ?etc. Treatment principles of abnormal Balgam disease is to first use the appropriate ?ripening agent (Munziq) to mature and accumulate abnormal fluid, then to excrete it ?by using the appropriate scavengers (Mushil) and in the end to recover the ?temperament (Mizaj) and the power(Kuwwat), balance body fluid, then lay the ?foundation for the treatment of disease. In this thesis, research was done on the ?preparation process ，chemical composition and quality control of abnormal Balgam ?Munzij and Mushil, and their biological activities in vivo and in vitro to provide the ?scientific basis for their development and utilization.? ?1. Preparation technology of Munziq and Mushil Munziq Preparation technology was as follows: it was prepared by the method of ?water extraction, the best extraction parameters were determined by the extraction ?yield and general flavone, single factor experiments and orthogonal experiments. The ?Rosa rugosa Thunb. follow a traditional method of Uyghur Medicine Soak extract , ?method was water for once. 20 times the water of its own weight , Soak temperature ?was 60 degrees lasted 6 hours. The rest prescription first Souk 24 hours , then reflux ?extraction with water for twice. 14 times the water of its own weight was Each time . ?Each reflux extraction lasted 1 hours. The fried liquid was gathered, then filtered.? Mushil Preparation technology was as follows: it was prepared by the method of ?water extraction, the best extraction parameters were determined by the extraction ?yield and general flavone, single factor experiments and orthogonal experiments. The ?Alhagi pseudoalhagi (M.B.) Desv. And Cassia fistula L. follow a traditional method ?of Uyghur Medicine each Herbs Soak extract , method was water for once. 10 times ?the water of its own weight , Soak temperature was 60 degrees lasted 4 hours. The ?rest prescription first Souk 24 hours , then reflux extraction with water for twice. 14 ?times the water of its own weight was Each time . Each reflux extraction lasted 1 ?hours. The fried liquid was gathered, then filtered. The filtrate was concentrated to a ?relative density of 1.01 Munziq and 1.04 Mushil, and then 2g sorbic acid was added ?and mixed . Finally we adjusted the pH to 4.0 to 6.0 and added water to 1000ml and ?steam sterilization.? ?2. Analysis by LC/QTOF–MS/MS and Chemical Compositions Chemical Compositions of Munziq and Mushil were separated and analysed by ?LC/QTOF–MS/MS in negative electrospray ionisation (ESI) mode at different ?collision energy (CE) values. The maximal structural information was obtained for the ?identi?cation of components. 24 compounds of Munziq and 21 compounds of Mushil, ?including organic acid, tannins and ?avonoids, were identi?ed or partially ?characterised according to accurate mass measurement and reference substance.? We used High Speed Counter-current Chromatography to separate compounds ?from the fraction extracted by ethyl acetate of Munziq and Mushil. Three compounds, ?Methyl gallateⅠ, 5,7,4'-tri-hydroxy-6，8-diprenylf isoflavonesⅡ, Glycyrol were ?isolated from of Munziq. Another two compounds, Cucurbitacins E-2-O-β-D –?Glucopyranosides Ⅳ, 2,5-Dimethyl-7-hydroxychromoneⅥ, were isolated from of ?Mushil. The chemical structures of compounds Ⅰ-Ⅵ were established on the basis of ?a spectroscopic analysis including 1H NMR and13C NMR.? ?3. Research on quality standards of Munziq and Mushil.? We have established quality standards of Munziq and Mushil. Routine inspection ?was in line with the 2010 version of "Chinese Pharmacopoeia" requirement. ?Glycyrrhiza uralensis Fisch., Rosa rugosa Thunb of Munziq, and Glycyrrhiza uralensis ?Fisch., Cassia fistula L.of Mushil were identificated by TLC. In addition, the HPLC ?method of determination the content of gallic acid、ellagic acids、glycyrrhizic acid was ?established.? ?4.Studies on the Biological Activities in vivo and in vitro ? We studied biological activity of Munziq and Mushil in vivo and in vitro. In ?vitro, we investigated the the inhibitory effects on protein tyrosine phosphatase ??1B(PTP1B)、α-glucosidase and human recombinant aldose(HAR) reductase and ?free radical scavenging experiments on ABTS and DPPH. The results showed that ?these samples displayed different inhibitory activity against PTP1B and α?-glucosidase. Howeve，they showed no activity against HAR. They still held great ?promise although the effects on radical scavenging were weak compared with vitamin ?C. The results of present study clearly demonstrated the potential of using for ?prevention and treatment of diabetes.? To observe the effect of Munziq and Mushil extract on glucose and ?lipid metabolism of type 2 diabetes rats and discuss its possible mechanisms .? Animal model of type 2 diabetes was established by a high fat diet with ?intraperitoneal injection of STZ into male SD rats and give low-dose, middle-dose, ?high-dose of 2 Samples extract. The level of Fasting plasma glucose (FPG), Fasting ?insulin (FINS), Total cholesterol (TC), Triglyceride (TG), High density lipoprotein ?cholesterol (HDL-C), Low density lipoprotein cholesterol (LDL-C), Insulin sensitivity ?index (ISI) and the pathological changes of the liver and kidney were evaluated.? Compared with model group, the liver index in Munziq extract group declined, ?the level of FPG declined and ISI increased—except low-dose group of Munziq, And ?dose of regularity. the level of TG,LHL-C of low-dose group of Munziq declined, ?HDL-C was rise . and the levels of TG, TC, LDL-C of all others Munziq extract group ?declined. HDL-C was on rise . Munziq extract group can lighten the pathological ?lesion of the liver and kidney.? Compared with model group, 5 weeks later the liver index in preparation ?process Mushil extract group declined, the level of FPG was decreased and ISI was ?increased in dose dependent manner. The level of TG,LHL-C of low-dose group of ?Mushil declined, HDL-C was increased. and the levels of TG, LDL-C of all others ?Mushil extract group declined, HDL-C was on rise .
|希尔艾力•吐尔逊. 异常黏液质成熟剂、清除剂的制备及其生物活性研究[D]. 北京. 中国科学院大学,2014.|
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