|关键词||兰州湾脆蘑 Its序列 培养特性 栽培驯化|
Lanzhouwan mushroom is loved by the local people because of fine fruit body morphology, delicious flavor and rich nutrition. In the autumn of 2012 the writer collected a large mushroom fruiting body from a shady cornfields at Xinjiang Manasi County Lanzhou town.The internal transcribed spacer of Lanzhouwan mushroom was sequenced after PCR amplification.This studies the biological characteristics on understanding its growth characteristics and ability to adapt to the existing cultivation conditions.The cultivation condition of Lanzhouwan mushroom mycelium were optimized by the experiments of Plackett-Burman design,the steepest ascent and orthogonal designs. Then this studies the wild mushroom artificial domestication and cultivation. The main results were as follows: 1. Molecular identification was carried out using rDNA-ITS sequence analysis. ITS sequence was amplified by universal primers ITS1 and ITS4. Sequence was measured 684bp fragments (GenBank accession number KF632894). ITS sequences of Lanzhouwan mushroom was compared with those registered in the DNA sequence database on Gen Bank,the identification results were obtained according to comparing other the homology sequence in DNA sequence database on Gen Bank. We got a sequence with accession number JF908330.1 of Lyophyllum decastes which homology was 99%. The Neighbor-Joining tree showed that Lanzhouwan mushroom identified Lyophyllum decastes. 2. The biological characteristics of vild Lanzhouwan mushroom mycelium was studied about five aspects of carbon source, nitrogen source,C/N, temperature and pH. The research on biological characters showed that mycelium grew well when brown sugar served as the carbon source, when bran was the nitrogen source, when C/N was 50:1, pH was 6.0 and when the temperature were 20-22.5℃. 3. The Plackett-Burman design showed that the main factors influencing mycelium growth were brown sugar,bran and KH2PO4. The optimum medium for Lanzhouwan mushroom mycelium growth was identified as Brown sugar 30 g/L + KH2PO4 0.5 g/L + Bran 3.0 g/L + MgSO4 0.5 g/L+ K2HPO4 0.5 g/L by experiments of the steepest ascent and orthogonal designs. On the optimum medium the colony diameter was increased from 56.50 mm to 72.25mm. 4. Cultivated on the wood flour substrate and wheat grain substrate, the spawn run period was long and there is no formation of primordium. While cultivated on the turf-grass,cottonseed hulls substrate and cornstalks substrate, the mycelium grew faster, primordia appeared earlier and fruit bodies of L. Decastes Formed. On the experiments of L. Decastes cultivated formulation, result displayed that L. Decastes could grow up on a substrate consisting of 490 g/kg~590 g/kg turf-grass, 300 g/kg~400 g/kg cottonseed hulls,100 g/kg bran and 10 g/kg lime.The cultivation cycle is 70-90 days and it can be cultivated for two cycles in one year.
|胥萌萌. 兰州湾脆蘑菌丝体培养特性及栽培驯化研究[D]. 北京. 中国科学院大学,2014.|
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