|关键词||罗布麻 总多酚 提取 纯化 降糖活性 降糖活性 抗氧化活性 化学成分。|
罗布麻叶是夹竹桃科罗布麻属植物罗布麻（Apocynum venetum L.）的干燥叶，药理活性研究表明，罗布麻叶具有降血压、降血脂、抗抑郁、抗氧化等作用，对糖尿病、肥胖症和心血管类等慢性疾病有一定的预防和改善作用。目前，尚未发现罗布麻化学成分中有关降血糖活性的研究报道。本论文旨在寻找富集其总多酚有效成分的方法，阐明其抗糖尿病作用的物质基础，以期开发一种具有抗氧化和抗糖尿病作用的罗布麻保健功能产品。主要内容如下：
The leaves of Apocynum venetum L. (AVL) is the dry leaves of Apocynum plants. Modern pharmacological studies has also indicated that the role of AVL in lowering blood pressure, antidepressant, antioxidation and so on. Researchers also have demonstrated that AVL could prevent or manage chronic health issues such as diabetes, obesity and cardiovascular disease. However, the hypoglycemic activity of chemical composition of AVL have not yet reported. Our goal was to discovery and enrich its effective ingredients of total polyphenols, and clarify its substance basis of antidiabetic activity. We hope that a functional foods of antidiabetic activity will be developed. The present study included as follow: 1. Study on the chemicial constituents of AVL. Six compounds were isolated, and identified by 1H-NMR and 13C-NMR. These compounds were isoquercetin, rutin, astragalin, quercetin, kaempferol, scopoletin. 2.Establish the process of extraction and purification method of total polyphenols of AVL. The preparation process of extracts was investigated by the single factor test and orthogonal experiment based on determination of the reference of gallic acid. The optimum extraction conditions were to extract with 15 times amount of 50 % ethanol by extract twice, and with the temperature of 70℃ by 2 hours each time. Then, the purification process of total polyphenols of the extract of AVL was studied. Ten kinds of different of macroporous resins for the separation and enrichment of total polyphenols were screened by static adsorption and desorption. The type of HPD-300 resin showed the best adsorption and desorption capacity for polyphenols than others, and abtained the optimum parameters for adsorption and desorption with HPD-300 through dynamic adsorption experiment.The optimum conditions of purification of sample concentration was 6.0 mg?mL-1, flow rate was 2 BV?h-1 and solution treatment capacity was 8 BV. The eluting agent as 5 BV purity water, with the total polyphenols could be desorbed by 3 BV of 50 % alcohol at the flow rate 2 BV?h-1. Ultimaately, The purity of total polyphenol increased from 19 % to 47 %. 3. Content analysis of chemicial composition, antioxidant and hypoglycemic activity of the effective part of total polyphenols. This part is rich in tannin (40 %) and flavonoids (49 %). The results showed that the effective part of total polyphenols had strong antioxidant activity and PTP-1B inhibitory activity. Those results suggested that its might be have anti-diabetes activity.
|石丽娟. 罗布麻叶化学成分及总多酚纯化工艺研究[D]. 北京. 中国科学院大学,2014.|
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