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雪莲原生质体的分离和培养方法
王晓军; 袁永娴; 郝秀英; 赵明安; 刘敏; 徐琴; 康喜亮
2011-01-05
Rights Holder中国科学院新疆理化技术研究所
Date Available2013-11-27
Country中国
Subtype发明
Abstract本发明涉及新疆雪莲原生质体的分离和培养方法,该方法由无菌雪莲苗的培养、胚性愈伤组织的诱导及分化、原生质的分离与纯化和原生质体培养四个步骤完成,均适用于新疆雪莲的原生质体的分离和培养。同时本发明具有方法简单易于操作,对设备和培养条件的要求较低;此外,酶解的方法实用,去除细胞壁条件温和,分离原生质体的得率高、活性强易于雪莲原生质体的培养和植株再生,为原生质体培养和融合奠定了基础,也为新疆雪莲种质资源创新和转基因雪莲的研究奠定了基础。
Application Date2009-09-30
Patent NumberZL200910113470.8
Status授权
Application Number200910113470.8
Document Type专利
Identifierhttp://ir.xjipc.cas.cn/handle/365002/3028
Collection资源化学研究室
Recommended Citation
GB/T 7714
王晓军,袁永娴,郝秀英,等. 雪莲原生质体的分离和培养方法. ZL200910113470.8[P]. 2011-01-05.
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