中国科学院新疆理化技术研究所机构知识库
Advanced  
XJIPC OpenIR  > 资源化学研究室  > 期刊论文
学科主题: General & Internal Medicine (provided by Thomson Reuters)
题名: 厚叶岩白菜组培苗、种子苗和野生苗不同部位有效成分含量分析
其他题名: effective components analysis in different parts of the tissue culture seedlings,seed seedlings and wild seedlings of bergenia crassifolia
作者: 刘敏; 王卉; 陈艳瑞; 努尔波拉提; 宁慧霞; 王晓军
关键词: 厚叶岩白菜 ; 组培再生植株 ; 岩白菜素 ; 儿茶素 ; 高效液相色谱
刊名: Lishizhen Medicine and Materia Medica Research
发表日期: 2013
卷: 24, 期:6, 页:1484-1486
收录类别: CSCD
资助者: 中国科学院西部之光西部博士项目; 国家自然科学基金资助项目; 中科院西部行动计划高新技术项目
摘要: 目的测定厚叶岩白菜组培再生植株、种子苗和野生苗不同部位岩白菜素和儿茶素的含量,为人工种植厚叶岩白菜的质量评价提供科学依据。方法采用反相C_(18 )色谱柱,甲醇-水为流动相梯度洗脱,流速为1.0 ml.min~(-1),检测波长为275 nm,对不同厚叶岩白菜苗的主根、须根和叶片等不同部位中的岩白菜素、儿茶素含量进行HPLC分析比较。结果厚叶岩白菜不同部位的岩白菜素、儿茶素含量存 在一定差异,主根和须根中岩白菜素的含量较高;但组培苗和种子苗与野生苗中的岩白菜素和儿茶素含量差别不大,甚至组培苗叶片岩白菜素含量高出种子苗和野生 苗1倍;而儿茶素的含量组培苗主根、须根均高于种子苗和野生苗含量3倍左右。结论该研究表明采用组培或种子人工种植的厚叶岩白菜其有效成分能够满足临床应 用的要求。
英文摘要: Objective To determine the bergenin and catechin contents in the tissue culture seedlings,seed seedlings and wild seedlings of Bergenia crassifolia,which would lay the foundation for the evaluation of artificial planting of this medical herb. Methods The HPLC analysis was achieved by using a C_(18) reverse column and methanol - water as the mobile phase,with a flow rate of 1. 0 ml·min~(-1) and detected by UV at 275nm. The contents of bergenin and catechin in the different parts of axial root,fibrous root and leaves of B. crassifolia. Results The contents of bergenin and catechin varies in different parts of,the content of bergenin is higher in axial root and fibrous root while the content of catechin is higher in leaves. However,there are no significant differences between tissue culture seedlings,seed seedlings and wild seedlings. Conclusion This study indicated that the effective components of the artificial planting seedlings could fulfill the reguirments of medical applications.
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/2856
Appears in Collections:资源化学研究室_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
厚叶岩白菜组培苗、种子苗和野生苗不同部位有效成分含量分析.pdf(188KB)期刊论文作者接受稿开放获取View 联系获取全文

作者单位: 中国科学院新疆理化技术研究所;新疆农业科学院生物质能源研究所

Recommended Citation:
刘敏,王卉,陈艳瑞,等. 厚叶岩白菜组培苗、种子苗和野生苗不同部位有效成分含量分析[J]. Lishizhen Medicine and Materia Medica Research,2013,24(6):1484-1486.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[刘敏]'s Articles
[王卉]'s Articles
[陈艳瑞]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[刘敏]‘s Articles
[王卉]‘s Articles
[陈艳瑞]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 厚叶岩白菜组培苗、种子苗和野生苗不同部位有效成分含量分析.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace