Objective To determine the bergenin and catechin contents in the tissue culture seedlings,seed seedlings and wild seedlings of Bergenia crassifolia,which would lay the foundation for the evaluation of artificial planting of this medical herb. Methods The HPLC analysis was achieved by using a C_(18) reverse column and methanol - water as the mobile phase,with a flow rate of 1. 0 ml·min~(-1) and detected by UV at 275nm. The contents of bergenin and catechin in the different parts of axial root,fibrous root and leaves of B. crassifolia. Results The contents of bergenin and catechin varies in different parts of,the content of bergenin is higher in axial root and fibrous root while the content of catechin is higher in leaves. However,there are no significant differences between tissue culture seedlings,seed seedlings and wild seedlings. Conclusion This study indicated that the effective components of the artificial planting seedlings could fulfill the reguirments of medical applications.