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Anti-inflammatory effects and hepatotoxicity of Tripterygium-loaded solid lipid nanoparticles on adjuvant-induced arthritis in rats
Xue Mei; Jiang Zhen-zhou; Wu Tao; Li Ji; Zhang Liang; Zhao Yan; Li Xue-jun; Zhang Lu-Yong; Yang Shu-yu
2012
Source PublicationPHYTOMEDICINE
ISSN0944-7113
Volume19Issue:11Pages:998-1006
AbstractTripterygium wilfordii Hook f. (TWHF) has been demonstrated to have anti-inflammatory, immunosuppressive effects and its clinical use was restricted to some extent due to some toxic effects on the digestive, urogenital, and blood circulatory systems, especially the male reproductive system. In the previous study, we had confirmed that TWHF-loaded solid lipid nanoparticles (SLN) have protective effects on male reproductive toxicity in rats. Anti-inflammatory effects and hepatotoxicity of TWHF-SLN remain to be unidentified. The present study was focused on the anti-inflammatory effect of complete Freund's adjuvant-induced arthritis in rats treated with TWHF-SLN as well as the effects of SLN delivery system on decreasing the hepatotoxicity induced by tripterygium. Sixty-four healthy male rats were randomly divided into eight groups with eight rats each. From day 18 after FCA injection, TWHF-SLN group (120, 60, 30 mg/kg) and TWHF group (120, 60, 30 mg/kg) were administered by oral gavage for 24 consecutive days. The control group was with saline and model control group was without any treatment. The volume of the right hind paws was evaluated at 0, 4, 8, 12, 18, 24, 30, 36 and 42 days post-injection of FCA by a home-made connected device. The serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transpeptidase (GGT), total bilirubin (TBIL) and albumin (ALB) levels were evaluated by an autoanalyzer. Activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) malondialdehyde (MDA) and xanthine oxidase (XOD) levels were determined using commercial kits. The PG level in sera was examined by double antibody sandwich method. Tissue histopathology was evaluated with hematoxylin and eosin (H&E). The results show that TWHF-SLN can significantly reduce rat paw volume at 60 mg/kg (p < 0.05) and PG levels in serum (p < 0.05); the levels of ALT, AST, ALP, GGT in serum and MDA, XOD, GSH-PX in liver were not significantly elevated. Histopathology observation found that free TWHF caused more serious damage to the liver than TWHF-SLN. These results revealed that SLN delivery system can enhance the anti-inflammatory activity of TWHF, and meanwhile has a protective effect against TWHF-induced hepatotoxicity. Crown Copyright (C) 2012 Published by Elsevier GmbH. All rights reserved.
KeywordTripterygium WilF.rdii Hook F. (twhF. Solid Lipid Nanoparticles (Sln) Anti-inflammatory Rheumatoid Arthritis (Ra) Hepatotoxicity
Subject AreaPlant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
DOI10.1016/j.phymed.2012.06.006
Indexed BySCI
WOS IDWOS:000309616600007
Citation statistics
Cited Times:32[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/2795
Collection省部共建新疆特有药用资源利用重点实验室
AffiliationChina Pharmaceut Univ, Jiangsu Ctr Drug Screening Lab, Nanjing 210009, Jiangsu, Peoples R China;Xiamen Univ, Affiliated Hosp 1, Xiamen Diabet Inst, Xiamen 361003, Peoples R China;Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Nanjing Univ Chinese Med, Dept Pharmacol, Nanjing 210046, Jiangsu, Peoples R China
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GB/T 7714
Xue Mei,Jiang Zhen-zhou,Wu Tao,et al. Anti-inflammatory effects and hepatotoxicity of Tripterygium-loaded solid lipid nanoparticles on adjuvant-induced arthritis in rats[J]. PHYTOMEDICINE,2012,19(11):998-1006.
APA Xue Mei.,Jiang Zhen-zhou.,Wu Tao.,Li Ji.,Zhang Liang.,...&Yang Shu-yu.(2012).Anti-inflammatory effects and hepatotoxicity of Tripterygium-loaded solid lipid nanoparticles on adjuvant-induced arthritis in rats.PHYTOMEDICINE,19(11),998-1006.
MLA Xue Mei,et al."Anti-inflammatory effects and hepatotoxicity of Tripterygium-loaded solid lipid nanoparticles on adjuvant-induced arthritis in rats".PHYTOMEDICINE 19.11(2012):998-1006.
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