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学科主题: Engineering
题名: Bioassay-guided separation and purification of water-soluble antioxidants from Carthamus tinctorius L. by combination of chromatographic techniques
作者: Li Lingnan; Yang Yi; Hou Xueling; Gu Dongyu; Hang Ba; Abdulla Rahima; Wu Guirong; Xin Xuelei; Aisa Haji Akber
通讯作者: Aisa, HA
关键词: Carthamus tinctorius L. ; Bioassay-guided separation ; Combination of chromatographic techniques ; High-speed counter-current chromatography ; Water-soluble antioxidants
刊名: SEPARATION AND PURIFICATION TECHNOLOGY
发表日期: 2013
DOI: 10.1016/j.seppur.2012.11.027
卷: 104, 期:2, 页:200-207
收录类别: SCI
资助者: Key Projects in the National Science & Technology Pillar Program 2011BAI05B05; High Tech Research and Development Program of Xinjiang 201010108; China National Funds for Distinguished Young Scientists 30925045
摘要: A combined chromatographic method using high-speed counter-current chromatography (HSCCC) and Sephadex LH-20 chromatography was established for bioassay-guided separation and purification of water-soluble antioxidants from Carthamus tinctorius L florets. The crude sample II obtained by an initial cleanup step on the AB-8 macroporous resin exhibited a potential ABTS radical cation scavenging activity with the SC50 value of 49.28 mu g/mL. The HSCCC separation was performed with a two-phase solvent system composed of n-butanol-0.1 mol/L HCl (1:1, v/v) at a flow rate of 1.2 mL/min. After a single run, 3 mg hydroxysafflor yellow A (HSYA) with 98% purity was separated as a major component from 20 mg of crude sample II. In order to increase the yield and investigate the minor components, the sample size of HSCCC separation was enlarged to 1 g. Then, HSCCC fractions were subjected to Sephadex LH-20 chromatography and eluted with distilled water at a flow rate of 1.5 mL/min to remove the acid and further separate the components. This separation yielded 184.0 mg of HSYA (1), 10.0 mg of 5,4'-dihydroxyflavone-3,6-di-O-beta-D-glucoside-7-O-beta-D-glucuronide (2), 3.0 mg of 3-O-caffeoylquinic acid (3), and 3.2 mg of 4-O-beta-D-glucosyl-trans-p-coumaric acid (4) from 1 g crude sample II of C tinctorius L. florets. The purity of the separated compounds were over 95% by HPLC analysis, and their chemical structures were confirmed by MS, H-1 NMR and C-13 NMR. Compounds 3 and 4 were found from this plant for the first time. Antioxidant activities assayed in vitro by ABTS radical cation scavenging showed that the SC50 values of the above four compounds were 44.39 +/- 1.62 mu g/mL, 78.13 +/- 1.00 mu g/mL, 21.85 +/- 1.96 mu g/mL, and 31.44 +/- 2.06 mu g/mL, respectively. (C) 2012 Elsevier B.V. All rights reserved.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/2682
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China;Xinjiang Med Univ, Sch Pharm, Urumqi 830054, Peoples R China

Recommended Citation:
Li Lingnan,Yang Yi,Hou Xueling,et al. Bioassay-guided separation and purification of water-soluble antioxidants from Carthamus tinctorius L. by combination of chromatographic techniques[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2013,104(2):200-207.
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