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Assessment of the estrogenic activities of chickpea (Cicer arietinum L) sprout isoflavone extract in ovariectomized rats
Ma Hai-rong; Wang Jie; Qi Hong-xue; Gao Yan-hua; Pang Li-juan; Yang Yi; Wang Zhen-hua; Duan Ming-jun; Chen Hua; Cao Xu; Aisa Haji Akber; Aisa, HA
2013
Source PublicationACTA PHARMACOLOGICA SINICA
ISSN1671-4083
Volume34Issue:3Pages:380-386
Abstract

Aim: Chickpea (Cicer arietinum L) is a traditional Uighur herb. In this study we investigated the estrogenic activities of the isoflavones extracted from chickpea sprouts (ICS) in ovariectomized rats.

Methods: Ten-week-old virgin Sprague-Dawley female rats were ovariectomized (OVX). The rats were administered via intragastric gavage 3 different doses of ICS (20, 50, or 100 mg.kg(-1).d(-1)) for 5 weeks. Their uterine weight and serum levels of 17 beta-estradiol (E-2), follicle stimulating hormone (FSH) and luteinizing hormone (LH) were measured. The epithelial height, number of glands in the uterus, and number of osteoclasts in the femur were histologically quantified, and the expression of proliferating cell nuclear antigen (PCNA) was assessed immunohistochemically. Bone structural parameters, including bone mineral density (BMD), bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp) were measured using Micro-CT scanning.

Results: Treatments of OVX rats with ICS (50 or 100 mg.kg(-1).d(-1)) produced significant estrogenic effects on the uteruses, including the increases in uterine weight, epithelial height and gland number, as well as in the expression of the cell proliferation marker PCNA. The treatments changed the secretory profile of ovarian hormones and pituitary gonadotropins: serum E-2 level was significantly increased, while serum LH and FSH levels were decreased compared with the vehicle-treated OVX rats. Furthermore, the treatments significantly attenuated the bone loss, increased BMD, BV/TV and Tb.Th and decreased Tb.Sp and the number of osteoclasts. Treatment of OVX rats with the positive control drug E-2 (0.25 mg.kg(-1).d(-1)) produced similar, but more prominent effects.

Conclusion: ICS exhibits moderate estrogenic activities as compared to E-2 in ovariectomized rats, suggesting the potential use of ICS for the treatment of menopausal symptoms and osteoporosis caused by estrogen deficiency.

KeywordChickpea Chickpea Sprout Isoflavone Phytoestrogen 17 Beta-estradiol Ovariectomized Rat Uterine Follicle Stimulating Hormone Luteinizing Hormone Osteoporosis
Subject AreaChemistry ; Pharmacology & Pharmacy
DOI10.1038/aps.2012.160
Indexed BySCI
WOS IDWOS:000315931300008
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/2671
Collection省部共建新疆特有药用资源利用重点实验室
Corresponding AuthorAisa, HA
AffiliationChinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Shihezi Univ, Coll Pharm, Minist Educ, Key Lab Xinjiang Endem Phytomed Resources, Shihezi 832002, Peoples R China;Shihezi Univ, Sch Med, Shihezi 832002, Peoples R China;Xinjiang Med Univ, Teaching Hosp 1, Ctr Expt Anim, Urumqi 830011, Peoples R China;Johns Hopkins Univ, Dept Orthopaed Surg, Sch Med, Baltimore, MD 21205 USA
Recommended Citation
GB/T 7714
Ma Hai-rong,Wang Jie,Qi Hong-xue,et al. Assessment of the estrogenic activities of chickpea (Cicer arietinum L) sprout isoflavone extract in ovariectomized rats[J]. ACTA PHARMACOLOGICA SINICA,2013,34(3):380-386.
APA Ma Hai-rong.,Wang Jie.,Qi Hong-xue.,Gao Yan-hua.,Pang Li-juan.,...&Aisa, HA.(2013).Assessment of the estrogenic activities of chickpea (Cicer arietinum L) sprout isoflavone extract in ovariectomized rats.ACTA PHARMACOLOGICA SINICA,34(3),380-386.
MLA Ma Hai-rong,et al."Assessment of the estrogenic activities of chickpea (Cicer arietinum L) sprout isoflavone extract in ovariectomized rats".ACTA PHARMACOLOGICA SINICA 34.3(2013):380-386.
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