Assessment of the estrogenic activities of chickpea (Cicer arietinum L) sprout isoflavone extract in ovariectomized rats
Ma Hai-rong; Wang Jie; Qi Hong-xue; Gao Yan-hua; Pang Li-juan; Yang Yi; Wang Zhen-hua; Duan Ming-jun; Chen Hua; Cao Xu; Aisa Haji Akber; Aisa, HA
2013
发表期刊ACTA PHARMACOLOGICA SINICA
ISSN1671-4083
卷号34期号:3页码:380-386
摘要

Aim: Chickpea (Cicer arietinum L) is a traditional Uighur herb. In this study we investigated the estrogenic activities of the isoflavones extracted from chickpea sprouts (ICS) in ovariectomized rats.

Methods: Ten-week-old virgin Sprague-Dawley female rats were ovariectomized (OVX). The rats were administered via intragastric gavage 3 different doses of ICS (20, 50, or 100 mg.kg(-1).d(-1)) for 5 weeks. Their uterine weight and serum levels of 17 beta-estradiol (E-2), follicle stimulating hormone (FSH) and luteinizing hormone (LH) were measured. The epithelial height, number of glands in the uterus, and number of osteoclasts in the femur were histologically quantified, and the expression of proliferating cell nuclear antigen (PCNA) was assessed immunohistochemically. Bone structural parameters, including bone mineral density (BMD), bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp) were measured using Micro-CT scanning.

Results: Treatments of OVX rats with ICS (50 or 100 mg.kg(-1).d(-1)) produced significant estrogenic effects on the uteruses, including the increases in uterine weight, epithelial height and gland number, as well as in the expression of the cell proliferation marker PCNA. The treatments changed the secretory profile of ovarian hormones and pituitary gonadotropins: serum E-2 level was significantly increased, while serum LH and FSH levels were decreased compared with the vehicle-treated OVX rats. Furthermore, the treatments significantly attenuated the bone loss, increased BMD, BV/TV and Tb.Th and decreased Tb.Sp and the number of osteoclasts. Treatment of OVX rats with the positive control drug E-2 (0.25 mg.kg(-1).d(-1)) produced similar, but more prominent effects.

Conclusion: ICS exhibits moderate estrogenic activities as compared to E-2 in ovariectomized rats, suggesting the potential use of ICS for the treatment of menopausal symptoms and osteoporosis caused by estrogen deficiency.

关键词Chickpea Chickpea Sprout Isoflavone Phytoestrogen 17 Beta-estradiol Ovariectomized Rat Uterine Follicle Stimulating Hormone Luteinizing Hormone Osteoporosis
学科领域Chemistry ; Pharmacology & Pharmacy
DOI10.1038/aps.2012.160
收录类别SCI
WOS记录号WOS:000315931300008
引用统计
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/2671
专题省部共建新疆特有药用资源利用重点实验室
通讯作者Aisa, HA
作者单位Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Shihezi Univ, Coll Pharm, Minist Educ, Key Lab Xinjiang Endem Phytomed Resources, Shihezi 832002, Peoples R China;Shihezi Univ, Sch Med, Shihezi 832002, Peoples R China;Xinjiang Med Univ, Teaching Hosp 1, Ctr Expt Anim, Urumqi 830011, Peoples R China;Johns Hopkins Univ, Dept Orthopaed Surg, Sch Med, Baltimore, MD 21205 USA
推荐引用方式
GB/T 7714
Ma Hai-rong,Wang Jie,Qi Hong-xue,et al. Assessment of the estrogenic activities of chickpea (Cicer arietinum L) sprout isoflavone extract in ovariectomized rats[J]. ACTA PHARMACOLOGICA SINICA,2013,34(3):380-386.
APA Ma Hai-rong.,Wang Jie.,Qi Hong-xue.,Gao Yan-hua.,Pang Li-juan.,...&Aisa, HA.(2013).Assessment of the estrogenic activities of chickpea (Cicer arietinum L) sprout isoflavone extract in ovariectomized rats.ACTA PHARMACOLOGICA SINICA,34(3),380-386.
MLA Ma Hai-rong,et al."Assessment of the estrogenic activities of chickpea (Cicer arietinum L) sprout isoflavone extract in ovariectomized rats".ACTA PHARMACOLOGICA SINICA 34.3(2013):380-386.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Assessment of the es(6793KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ma Hai-rong]的文章
[Wang Jie]的文章
[Qi Hong-xue]的文章
百度学术
百度学术中相似的文章
[Ma Hai-rong]的文章
[Wang Jie]的文章
[Qi Hong-xue]的文章
必应学术
必应学术中相似的文章
[Ma Hai-rong]的文章
[Wang Jie]的文章
[Qi Hong-xue]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Assessment of the estrogenic activities of chickpea (Cicer arietinum L) sprout isoflavone extract in ovariectomized rats.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。