中国科学院新疆理化技术研究所机构知识库
Advanced  
XJIPC OpenIR  > 省部共建新疆特有药用资源利用重点实验室  > 期刊论文
学科主题: Pharmacology & Pharmacy
题名: The Estrogenic Activity of Isoflavones Extracted from Chickpea Cicer arietinum L Sprouts in Vitro
作者: Ma HaiRong; Wei HuaBo; Chen Zhen; Yang Yi; Wang ZhengHua; Madina Habasi; Cao Xu; Akber Aisa Haji
关键词: Cicer arietinum L sprouts ; isoflavones ; estrogenic activity
刊名: PHYTOTHERAPY RESEARCH
发表日期: 2013
DOI: 10.1002/ptr.4858
卷: 27, 期:8, 页:1237-1242
收录类别: SCI
资助者: National Natural Science Foundation of China 81102890; CAS/SAFEA International Partnership Program for Creative Research Teams KGCX2-YW-503; Natural Science Foundation of Xinjiang Province, China 2010211A58; China National Science Fund for Distinguished Young Scholars 30925045
摘要: Isoflavones have drawn attention due to their potential therapeutic use. Isoflavones are the important chemical components of the seeds and sprouts of chickpea and higher isoflavones in sprouts than in seeds. However, there have been no previous reports of the estrogenic activity of isoflavones extracted from chickpea Cicer arietinum L sprouts (ICS) in vitro. In this study, which incorporated several in vitro bioassays methods, we systematically evaluated the estrogenic properties of ICS. MTT assay showed that ICS at the low concentration ranges (10(-3)-1mg/L) promoted MCF-7 cell growth, while at high concentrations, (>1mg/L) inhibited cell proliferation, indicating ICS worked at a diphasic mechanism. Flow cytometric analysis further calculated the proliferation rate of ICS at low concentration (1mg/L). ER/Luc trans-activation assay and then semi-quantitative RT-PCR analysis indicated that ICS at low concentrations induced ER-mediated luciferase activity in MCF-7 cells and promoted the ER downstream target gene pS2 and PR trans-activation. These effects were inhibited by ICI 182,780, a special antagonist of ER, indicating that an ER-mediating pathway was involved. Alkaline phosphatase (AP) expression in Ishikawa cells showed that ICS at low concentrations stimulated AP expression. Our current study is the first to demonstrate that ICS has significant estrogenic activity in vitro. ICS may be useful as a supplement to hormone replacement therapy and in dietary supplements. Copyright (c) 2012 John Wiley & Sons, Ltd.
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/2643
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
The Estrogenic Activity of Isoflavones Extracted from Chickpea Cicer arietinum L Sprouts in Vitro.pdf(228KB)期刊论文作者接受稿开放获取View 联系获取全文

作者单位: Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Peoples Hosp Fengdu Cty Chongqing City, Chongqing 408200, Peoples R China;Shihezi Univ, Coll Pharm, Minist Educ, Key Lab Xinjiang Endem Phytomed Resources, Shihezi 832002, Peoples R China;Johns Hopkins Univ, Sch Med, Dept Orthopaed Surg, Baltimore, MD 21205 USA

Recommended Citation:
Ma HaiRong,Wei HuaBo,Chen Zhen,et al. The Estrogenic Activity of Isoflavones Extracted from Chickpea Cicer arietinum L Sprouts in Vitro[J]. PHYTOTHERAPY RESEARCH,2013,27(8):1237-1242.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ma HaiRong]'s Articles
[Wei HuaBo]'s Articles
[Chen Zhen]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ma HaiRong]‘s Articles
[Wei HuaBo]‘s Articles
[Chen Zhen]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: The Estrogenic Activity of Isoflavones Extracted from Chickpea Cicer arietinum L Sprouts in Vitro.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace