The Estrogenic Activity of Isoflavones Extracted from Chickpea Cicer arietinum L Sprouts in Vitro
Ma HaiRong; Wei HuaBo; Chen Zhen; Yang Yi; Wang ZhengHua; Madina Habasi; Cao Xu; Akber Aisa Haji
2013
发表期刊PHYTOTHERAPY RESEARCH
ISSN0951-418X
卷号27期号:8页码:1237-1242
摘要

Isoflavones have drawn attention due to their potential therapeutic use. Isoflavones are the important chemical components of the seeds and sprouts of chickpea and higher isoflavones in sprouts than in seeds. However, there have been no previous reports of the estrogenic activity of isoflavones extracted from chickpea Cicer arietinum L sprouts (ICS) in vitro. In this study, which incorporated several in vitro bioassays methods, we systematically evaluated the estrogenic properties of ICS. MTT assay showed that ICS at the low concentration ranges (10(-3)-1mg/L) promoted MCF-7 cell growth, while at high concentrations, (>1mg/L) inhibited cell proliferation, indicating ICS worked at a diphasic mechanism. Flow cytometric analysis further calculated the proliferation rate of ICS at low concentration (1mg/L). ER/Luc trans-activation assay and then semi-quantitative RT-PCR analysis indicated that ICS at low concentrations induced ER-mediated luciferase activity in MCF-7 cells and promoted the ER downstream target gene pS2 and PR trans-activation. These effects were inhibited by ICI 182,780, a special antagonist of ER, indicating that an ER-mediating pathway was involved. Alkaline phosphatase (AP) expression in Ishikawa cells showed that ICS at low concentrations stimulated AP expression. Our current study is the first to demonstrate that ICS has significant estrogenic activity in vitro. ICS may be useful as a supplement to hormone replacement therapy and in dietary supplements.

关键词Cicer Arietinum l Sprouts Isoflavones Estrogenic Activity
学科领域Pharmacology & Pharmacy
DOI10.1002/ptr.4858
收录类别SCI
WOS记录号WOS:000322114900020
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/2643
专题省部共建新疆特有药用资源利用重点实验室
作者单位Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Xinjiang Key Lab Plant Resources & Nat Prod Chem, Urumqi 830011, Peoples R China;Peoples Hosp Fengdu Cty Chongqing City, Chongqing 408200, Peoples R China;Shihezi Univ, Coll Pharm, Minist Educ, Key Lab Xinjiang Endem Phytomed Resources, Shihezi 832002, Peoples R China;Johns Hopkins Univ, Sch Med, Dept Orthopaed Surg, Baltimore, MD 21205 USA
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Ma HaiRong,Wei HuaBo,Chen Zhen,et al. The Estrogenic Activity of Isoflavones Extracted from Chickpea Cicer arietinum L Sprouts in Vitro[J]. PHYTOTHERAPY RESEARCH,2013,27(8):1237-1242.
APA Ma HaiRong.,Wei HuaBo.,Chen Zhen.,Yang Yi.,Wang ZhengHua.,...&Akber Aisa Haji.(2013).The Estrogenic Activity of Isoflavones Extracted from Chickpea Cicer arietinum L Sprouts in Vitro.PHYTOTHERAPY RESEARCH,27(8),1237-1242.
MLA Ma HaiRong,et al."The Estrogenic Activity of Isoflavones Extracted from Chickpea Cicer arietinum L Sprouts in Vitro".PHYTOTHERAPY RESEARCH 27.8(2013):1237-1242.
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