木犀草素对人胃癌裸鼠移植瘤的抑制效应及其机制
其他题名inhibitory effects of luteolin on human gastric carcinoma xenografts in nude mice and its mechanism
陆雪莹;   李艳红;   肖向文;   李晓波
2013
发表期刊中华医学杂志
ISSN0376-2491
卷号93期号:2页码:142-146
摘要

目的评价木犀草素对人胃癌BGC-823细胞裸鼠移植瘤的作用及其作用机制。方法建立BGC-823胃癌裸鼠移植瘤模型,裸鼠皮下接种BGC-823细胞 ,成瘤后,根据裸鼠体重,进行区组随机化分组,共分成5组(各8只):对照组(生理盐水,0.2 ml/只),5氟尿嘧啶组(30 mg/kg),木犀草素低(10 mg/kg) 、中(20 mg/kg) 、高(40 mg/kg)剂量组;各组给药期间测定瘤体积,绘制肿瘤生长曲线;各组用药结束后处死,称瘤重,计算抑瘤率,比较各组荷瘤鼠瘤重的差异;利用HE染色观察 肿瘤形态学变化,免疫组化检测肿瘤血管内皮生长因子A(VEGF-A)及基质金属蛋白酶9(MMP-9)表达变化。结果肿瘤生长曲线结果表明,5氟尿嘧啶 组,木犀草素低、中、高剂量组的肿瘤均有不同程度被抑制;木犀草素高剂量组肿瘤重量显著低于对照组[(0.290.01)比(0.380.03) g, P <0.01],抑瘤率达到24.87%;HE染色结果表明,随着木犀草素剂量的增大,瘤体坏死灶边缘有较多淋巴细胞浸润,且坏死区面积有增大趋势;免疫组 化结果显示:中、高剂量组VEGF-A阳性细胞积分分别为1.250.17和1.000.07,均明显低于对照组(1.500.15,均P < 0.05); 高剂量组MMP-9阳性细胞积分亦显著低于对照组(3.751.43比9.001.08, P < 0.01) 。结论木犀草素在体内可抑制肿瘤生长,其主要机制是可刺激体内免疫反应,同时抑制肿瘤细胞的VEGF-A、MMP-9蛋白表达。

其他摘要

Objective To explore the in vivo anticancer effects of luteolin with BGC-823 gastric carcinoma xenografts in nude mice and elucidate its mechanism. Methods After modeling of gastric carcinoma xenografts in nude mice, 40 BALB/c (nu/nu) nude mice were randomly divided into 5 groups(n=8 each). And an intraperitoneal injection of luteolin was administered at 10 mg/kg (low-dose), 20 mg/kg (middle-dose) and 40 mg/kg (high-dose) groups. And 5-fluorouracil (30 mg/kg) and control groups were also established. The growth curves of xenografts in nude mice were drawn and weight inhibition rates measured. The morphological features were detected by hematoxylin and eosin staining. And the protein expression levels of vascular endothelial growth factor A(VEGF-A) and matrix metalloproteinase 9 (MMP-9) were measured by immunohistochemistry. Results In vivo tumor formation test showed that tumor volume in nude mice treated with luteolin was smaller than that of control group. Tumor weights of high-dose luteoin group were lighter than those of the control ((0.290.01) vs (0.380.03) g). And the difference was statistically significant (P < 0.01). The rate of tumor inhibition in high-dose luteoin group was up to 24.87%. Lymphocytic invasion of tumor tissue was observed under light microscope in the treatment groups. Results of immunohistochemistry showed the positive cell integral of VEGF in middle and high-dose luteoin groups were 1.250.17 and 1.000.07 respectively. Both were significantly lower than that of control group (1.500.15, both P < 0.05). The positive cell integral of MMP-9 in high-dose luteoin group was markedly lower than that of control group (3.751.43 vs 9.001.08, P < 0.01). Conclusions Luteolin can effectively inhibit the in vivo growth of gastric tumor. The mechanism may be correlated with the stimulation of immune response and the down-regulated expressions of VEGF-A and MMP-9.

关键词木犀草素 胃肿瘤 血管内皮生长因子a 基质金属蛋白酶9
学科领域General & Internal Medicine (Provided By Thomson Reuters)
收录类别CSCD
CSCD记录号CSCD:4732200
引用统计
被引频次:3[CSCD]   [CSCD记录]
文献类型期刊论文
条目标识符http://ir.xjipc.cas.cn/handle/365002/2593
专题省部共建新疆特有药用资源利用重点实验室
作者单位中国科学院新疆理化技术研究所 中国科学院干旱区植物资源化学重点实验室
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陆雪莹; 李艳红; 肖向文; 李晓波. 木犀草素对人胃癌裸鼠移植瘤的抑制效应及其机制[J]. 中华医学杂志,2013,93(2):142-146.
APA 陆雪莹; 李艳红; 肖向文; 李晓波.(2013).木犀草素对人胃癌裸鼠移植瘤的抑制效应及其机制.中华医学杂志,93(2),142-146.
MLA 陆雪莹; 李艳红; 肖向文; 李晓波."木犀草素对人胃癌裸鼠移植瘤的抑制效应及其机制".中华医学杂志 93.2(2013):142-146.
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