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Alternative Titleeffects of icariin and icaritin on proliferation of breast cancer t47d cells
王洁;   陈花;   买迪娜;   马海蓉;   王振华;   李一
Source Publication中草药

目的研究淫羊藿苷及淫羊藿素对乳腺癌T47D细胞增殖的影响,探讨雌激素受体(ER)与其作用机制的关系。方法用不同浓度的淫羊藿苷和淫羊藿素处理雌激素 受体呈阳性的乳腺癌T47D细胞系后,MTT法检测T47D细胞增殖,流式细胞术检测T47D细胞周期的变化,Western blotting法检测T47D细胞ERalpha和ERbeta蛋白表达。结果淫羊藿苷和淫羊藿素浓度为1*10~(-9)~1*10~(-6)mol /L,能显著促进T47D细胞增殖,且该作用可被雌激素受体拮抗剂ICI 182.780(1*10~(-6)mol/L)所拮抗。与对照组相比,淫羊藿苷和淫羊藿素1*10~(-7)mol/L使S期T47D细胞比例明显增加 ,增殖指数显著升高,但该作用可被ICI 182.780抑制。淫羊藿苷和淫羊藿素1*10~(-7)mol/L还可显著增加ERalpha、ERbeta蛋白表达(P<0.01)。结论淫羊藿苷 和淫羊藿素均可显著促进T47D细胞增殖,该作用可能是通过上调胞内ERalpha、ERbeta蛋白表达介导的。

Other Abstract

Objective To investigate the effects of icariin (Ica) and icaritin (Ict) on the proliferationin of breast cancer T47D cells in vitro and to explore the relationship between estrogen receptor (ER) and the mechanisms. Methods The ER positive T47D cells were treated with Ica and Ict at different concentration. The proliferation of T47D cells was measured by MTT method, and the cell cycle changes during the proliferation were further evaluated using flow cytometric assay. The effects of Ica and Ict on the expression of ERalpha and ERbeta in T47D cells were analyzed by Western blotting. Results Ica and Ict (10~(?9)10~(?6) mol/L) stimulated the proliferation of T47D cells, and the proliferation was inhibited by the estrogen antagonist ICI 182.780 (10~(?6) mol/L). Compared with the control group, Ica and Ict (10~(?7) mol/L) could increase the amount of T47D cells arrested in S phase, and the stimulation was inhibited by ICI 182.780. As well Ica and Ict (10~(?7) mol/L) could up-regulate the expression of ERalpha and ERbeta proteins (P < 0.01). Conclusion Ica and Ict stimulate the proliferation of T47D cells, and the effect may be performed through up-regulating the expression of ERalpha and ERbeta proteins.

Keyword淫羊藿苷 淫羊藿素 乳腺癌t47d细胞 细胞增殖 雌激素受体
Subject AreaGeneral & Internal Medicine ; Pharmacology & Pharmacy (Provided By Thomson Reuters)
Indexed ByCSCD
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Cited Times:4[CSCD]   [CSCD Record]
Document Type期刊论文
Affiliation石河子大学药学院新疆特种植物药资源教育部重点实验室; 中国科学院新疆理化技术研究所干旱地区植物资源化学重点实验室
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GB/T 7714
王洁; 陈花; 买迪娜; 马海蓉; 王振华; 李一. 淫羊藿苷和淫羊藿素对乳腺癌T47D细胞增殖的影响[J]. 中草药,2013,44(11):1470-1475.
APA 王洁; 陈花; 买迪娜; 马海蓉; 王振华; 李一.(2013).淫羊藿苷和淫羊藿素对乳腺癌T47D细胞增殖的影响.中草药,44(11),1470-1475.
MLA 王洁; 陈花; 买迪娜; 马海蓉; 王振华; 李一."淫羊藿苷和淫羊藿素对乳腺癌T47D细胞增殖的影响".中草药 44.11(2013):1470-1475.
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