XJIPC OpenIR  > 资源化学研究室
小麦TaNADP-ME2基因重组植物表达载体构建及对水稻的遗传转化
Alternative Titlethe construction of plant overexpression vector of wheat tanadp-me2 gene and genetic transformation of rice
付振艳; 苟小清; 张正斌; 肖向文; 王晓军
2013
Source Publication华北农学报
ISSN1000-7091
Volume28Issue:3Pages:58-61
Abstract

小麦TaNADP-ME2是一光反应基因,并对干旱、盐、低温等非生物胁迫作出响应。构建TaNADP-ME2基因的重组植物表达载体,并转化水稻获得转 基因植株。利用重组技术构建植物表达载体,冻融法转化农杆菌,农杆菌介导方法转化水稻成熟胚诱导的愈伤组织,潮霉素筛选和PCR鉴定转基因植株。成功构建 了重组植物表达质粒pSUE_2,并将pSUE_2转入农杆菌EHA105,潮霉素磷酸转移酶(HPT)基因和TaNADP-ME2基因PCR鉴定获得1 6棵转基因阳性水稻植株。为进一步确定TaNADP-ME2基因的水分利用效率功能奠定了基础。

Other Abstract

The wheat TaNADP-ME2 was not only responsive to light but also to drought,salt and low temperature.The recombinant plant overexpression vector was constructed,transformed into rice and acquired transgenic rice plant.The vector was constructed by recombinant technology.The agrobacterium was transformed by freeze-thaw method.The callus induced from the mature embryo of rice was transformed by agrobacterium-mediated method.The transgenic rice was identified by hygromycin and PCR method.The results revealed that recombinant plant overexpression vector pSUE2 has been constucted succeedingly and transformed into EHA105.The 16 transgenic rice positive plants were acquired by the PCR detection from HPT and TaNADP-ME2 gene.This research laid a foundation for identification the function of TaNADP-ME2 gene in enhancing water use efficiency in drought tolerance.

Keyword农杆菌 基因转化 水稻
Subject AreaGenetics & Heredity (Provided By Thomson Reuters)
Indexed ByCSCD
CSCD IDCSCD:4884453
Citation statistics
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/2581
Collection资源化学研究室
Affiliation中国科学院新疆理化技术研究所;中国科学院遗传与发育生物学研究所农业资源中心
Recommended Citation
GB/T 7714
付振艳,苟小清,张正斌,等. 小麦TaNADP-ME2基因重组植物表达载体构建及对水稻的遗传转化[J]. 华北农学报,2013,28(3):58-61.
APA 付振艳,苟小清,张正斌,肖向文,&王晓军.(2013).小麦TaNADP-ME2基因重组植物表达载体构建及对水稻的遗传转化.华北农学报,28(3),58-61.
MLA 付振艳,et al."小麦TaNADP-ME2基因重组植物表达载体构建及对水稻的遗传转化".华北农学报 28.3(2013):58-61.
Files in This Item:
File Name/Size DocType Version Access License
小麦TaNADP-ME2基因重组植物表达(426KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[付振艳]'s Articles
[苟小清]'s Articles
[张正斌]'s Articles
Baidu academic
Similar articles in Baidu academic
[付振艳]'s Articles
[苟小清]'s Articles
[张正斌]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[付振艳]'s Articles
[苟小清]'s Articles
[张正斌]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 小麦TaNADP-ME2基因重组植物表达载体构建及对水稻的遗传转化.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.