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A sample pretreatment strategy for pH-zone-refining counter-current chromatography
Yang Yi; Gu Dongyu; Chen Qibin; Xin Xuelei; Yili Abulimiti; Aisa Haji Akber
2013
Source PublicationJOURNAL OF CHROMATOGRAPHY A
ISSN0021-9673
Volume1273Issue:1Pages:44-48
Abstract

A sample pretreatment method using acid base extraction was established for pH-zone-refining countercurrent chromatography (CCC) to increase the yield of the target compound. As the most important step, selection of two-phase solvent system for pH-zone-refining CCC should follow some rules. One of these rules is that when pH of two-phase solvent system is around 2 (for an acidic analyte) or 10 (for a basic analyte), partition coefficient values of K-acid or K-base should be much greater than 1. According to this rule, a new pretreatment method was developed that could substantially increase the yield of the target compound in pH-zone-refining CCC. The separation of the crude alkaloids extracted from the seeds of Sophora alopecuroides was selected as an example to show the advantage of this method. Before CCC separation, 20g of crude sample was extracted 3 times with the selected two-phase solvent system composed of MtBE-CH3CN-H2O (2:2:3, v/v), where pH of the upper phase was adjusted with triethylamine to 10. A log amount of the second basic extract thus obtained was subjected to pH-zone-refining CCC separation. As a result, 169 mg of sophoramin and 696 mg of sophoearpin were isolated from the second basic extract, where the yields have been doubled compared with those of the untreated sample (crude sample). Experimental results showed that the present method for pretreating the crude sample could produce substantially higher yields of target compounds in pH-zone-refining CCC.

KeywordSample Pretreatment Ph-zone-refining Counter-current Chromatography Sample Size Sample Recovery
Subject AreaBiochemistry & Molecular Biology ; Chemistry
DOI10.1016/j.chroma.2012.11.049
Indexed BySCI
WOS IDWOS:000314258100006
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.xjipc.cas.cn/handle/365002/2568
Collection省部共建新疆特有药用资源利用重点实验室
资源化学研究室
Corresponding AuthorAisa Haji Akber
AffiliationChinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China
Recommended Citation
GB/T 7714
Yang Yi,Gu Dongyu,Chen Qibin,et al. A sample pretreatment strategy for pH-zone-refining counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1273(1):44-48.
APA Yang Yi,Gu Dongyu,Chen Qibin,Xin Xuelei,Yili Abulimiti,&Aisa Haji Akber.(2013).A sample pretreatment strategy for pH-zone-refining counter-current chromatography.JOURNAL OF CHROMATOGRAPHY A,1273(1),44-48.
MLA Yang Yi,et al."A sample pretreatment strategy for pH-zone-refining counter-current chromatography".JOURNAL OF CHROMATOGRAPHY A 1273.1(2013):44-48.
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