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学科主题: Biochemistry & Molecular Biology ; Chemistry
题名: A sample pretreatment strategy for pH-zone-refining counter-current chromatography
作者: Yang Yi; Gu Dongyu; Chen Qibin; Xin Xuelei; Yili Abulimiti; Aisa Haji Akber
通讯作者: Aisa, HA
关键词: Sample pretreatment ; pH-zone-refining counter-current ; chromatography ; Sample size ; Sample recovery
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2013
DOI: 10.1016/j.chroma.2012.11.049
卷: 1273, 期:1, 页:44-48
收录类别: SCI
摘要: A sample pretreatment method using acid base extraction was established for pH-zone-refining countercurrent chromatography (CCC) to increase the yield of the target compound. As the most important step, selection of two-phase solvent system for pH-zone-refining CCC should follow some rules. One of these rules is that when pH of two-phase solvent system is around 2 (for an acidic analyte) or 10 (for a basic analyte), partition coefficient values of K-acid or K-base should be much greater than 1. According to this rule, a new pretreatment method was developed that could substantially increase the yield of the target compound in pH-zone-refining CCC. The separation of the crude alkaloids extracted from the seeds of Sophora alopecuroides was selected as an example to show the advantage of this method. Before CCC separation, 20g of crude sample was extracted 3 times with the selected two-phase solvent system composed of MtBE-CH3CN-H2O (2:2:3, v/v), where pH of the upper phase was adjusted with triethylamine to 10. A log amount of the second basic extract thus obtained was subjected to pH-zone-refining CCC separation. As a result, 169 mg of sophoramin and 696 mg of sophoearpin were isolated from the second basic extract, where the yields have been doubled compared with those of the untreated sample (crude sample). Experimental results showed that the present method for pretreating the crude sample could produce substantially higher yields of target compounds in pH-zone-refining CCC. (C) 2012 Elsevier B.V. All rights reserved.
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内容类型: 期刊论文
URI标识: http://ir.xjipc.cas.cn/handle/365002/2568
Appears in Collections:新疆维吾尔自治区植物资源化学重点实验室 _期刊论文

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作者单位: Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China

Recommended Citation:
Yang Yi,Gu Dongyu,Chen Qibin,et al. A sample pretreatment strategy for pH-zone-refining counter-current chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1273(1):44-48.
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